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M1544

Resorufin methyl ether

Name: Resorufin methyl ether
Brand: SIGMA

Synonym(s):

7-Methoxy-3H-phenoxazin-3-one, Methoxyresorufin, O⁷-Methylresorufin

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About This Item

Empirical Formula (Hill Notation):

C₁₃H₉NO₃

CAS Number:

5725-89-3

Molecular Weight:

227.22

UNSPSC Code:

12161501

NACRES:

NA.47

PubChem Substance ID:

24896665

MDL number:

MFCD00037663

Beilstein/REAXYS Number:

209529

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Properties

assay

≥98.0% (HPLC)

Quality Level

200

form

powder

mp

≥220 °C (lit.)

solubility

dichloromethane: 0.95-1.05 mg/mL, clear, orange to very deep orange

storage temp.

2-8°C

SMILES string

COc1ccc2N=C3C=CC(=O)C=C3Oc2c1

InChI

1S/C13H9NO3/c1-16-9-3-5-11-13(7-9)17-12-6-8(15)2-4-10(12)14-11/h2-7H,1H3

InChI key

KNYYMGDYROYBRE-UHFFFAOYSA-N

Description

Application

Resorufin methyl ether have been used as substrate in the incubation mixture, during the determination of cytochrome P4501A activities such as ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase(MROD) in liver microsomes, using high performance liquid chromatography(HPLC).

Biochem/physiol Actions

Fluorimetric substrate for cytochrome P450 linked enzymes.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Determination of cytochrome P450 1A activities in mammalian liver microsomes by high-performance liquid chromatography with fluorescence detection

Hanioka N, et al.

Journal of Chromatography. B, Biomedical Sciences and Applications, 744(2), 399-406 (2000)

Dissecting the insecticide-resistance- associated cytochrome P450 gene Cyp6g1.

Caroline McCart et al.

Pest management science, 64(6), 639-645 (2008-03-14)

The cytochrome P450 gene Cyp6g1 is overtranscribed in all field isolates of DDT-resistant Drosophila melanogaster (Meigen) and confers a fitness advantage when inherited via the female. Overtranscription is associated with the insertion of an Accord transposable element into the 5'