Chiral separations by capillary electrophoresis using proteins as chiral selectors.

Capillary electrophoresis (CE) methods using proteins as the chiral selectors have been developed for the separation of enantiomeric mixtures. For chiral separations in protein-based CE, two methods were utilized. One is affinity capillary electrochromatography (ACEC), and the other is affinity CE (ACE). This chapter deals with the advantages and disadvantages of ACEC and ACE. Furthermore, enantioseparations utilizing ACEC based on packed α(1)-acid glycoprotein-immobilized silica gels, immobilized avidin to fused silica capillaries and ACE based on penicillin G-acylase dissolved in the running buffer are described in detail.