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69966

MRS Broth

NutriSelect® Basic, powder, for Lactobacillus spp., for Bifidobacterium spp., pack of 500 g

Synonym(s):

Lactobacillus Broth acc. to De Man, Rogosa and Sharpe

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Basic
Ph:
6.2±0.2
Form:
powder
Suitability:
Bifidobacterium spp., Lactobacillus spp.
Application(s):
agriculture
bioburden testing
environmental
food and beverages
microbiology
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sterility

non-sterile

Quality Segment

100

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dipotassium hydrogen phosphate, 2 g/L , glucose, 20 g/L , magnesium sulfate monohydrate, 0.2 g/L , manganous sulfate tetrahydrate, 0.05 g/L , meat extract, 8 g/L , peptone, 10 g/L , sodium acetate, 5 g/L , triammonium citrate, 2 g/L , yeast extract, 4 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

pH

6.2±0.2

application(s)

agriculture
bioburden testing
environmental
food and beverages
microbiology

suitability

Bifidobacterium spp., Lactobacillus spp.

General description

MRS Broth is used for the enrichment, cultivation, and isolation of all species of Lactobacillus from all types of material according to DeMan, Rogosa, and Sharpe. The medium can be used for confirmatory tests of colonies isolated from MRS Agar. The MRS culture media contain polysorbate (Tween 80), acetate, magnesium, and manganese which are known to act as special growth factors for Lactobacilli as well as a rich nutrient base. As these media show a very low degree of selectivity Pediococcus and Leuconostoc species, as well as other secondary bacteria, may grow on them. Most of the accompanying microflora can be inhibited by thallium acetate, sorbic acid, acetic acid, sodium nitrite, cycloheximide, and polymyxin. These substances can be used at varying concentrations and combinations, but inevitably a compromise must be reached between selectivity and recovery rate of the organism.

Application

MRS Broth is used for the enrichment, cultivation and isolation of all species of Lactobacillus from all types of material

Preparation Note

Dissolve 51.25 g in 1 litre distilled water and add 1 ml Tween® 80 (Cat. No. P8074). Boil to dissolve the medium completely. Fill into containers and sterilize by autoclaving at 121°C for 15 minutes. lncubate the culture up to 3 days at 35°C or up to 5 days at 30°C. If possible, incubate the culture in a CO2 enriched atmosphere in an anaerobic jar.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Disclaimer

This product will be discontinued in the near future; however, stock is still available. The recommended replacement product is 1.10661 - MRS broth
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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