CMC0014
BL21(DE3) Chemically Competent Cells
Escherichia coli, rod shaped
Synonym(s):
Competent E. coli cells
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About This Item
UNSPSC Code:
41106202
NACRES:
NA.85
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped
Product Name
BL21(DE3) Chemically Competent Cells, for protein expression
biological source
Escherichia coli
grade
Molecular Biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Application
The E. coli strain BL21 (DE3) has been used:
- to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
- BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
- to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.
Features and Benefits
- a transformation efficiency of >1 × 107 cfu/μg.
- economical prices
- offering superior value for everyday protein expression work.
Other Notes
- BL21(DE3) chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
Storage Class
10 - Combustible liquids
wgk
WGK 2
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