- Affinity purification of MLL3/MLL4 histone H3K4 methyltransferase complex.
Affinity purification of MLL3/MLL4 histone H3K4 methyltransferase complex.
Methods in molecular biology (Clifton, N.J.) (2011-11-25)
Young-Wook Cho, Sunhwa Hong, Kai Ge
PMID22113294
ABSTRACT
Methylation on histone H3 lysine 4 (H3K4) correlates with actively transcribed genes. In mammalian cells, there exist multiple Set1-like histone H3K4 methyltransferase complexes, which have overlapping but distinct subunit compositions. Developing methods to isolate each of these histone H3K4 methyltransferase complexes would help understand the molecular mechanisms by which histone H3K4 methylation regulates mammalian gene expression. In this chapter, we provide a one-step affinity purification protocol on isolation of the MLL3/MLL4 histone H3K4 methyltransferase complex using FLAG-tagged PA1, a unique subunit of the MLL3/MLL4 complex.
MATERIALS
Product Number
Brand
Product Description
Millipore
ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solution
Sigma-Aldrich
ANTI-FLAG® M2 antibody, Mouse monoclonal, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)