How does the SNAP i.d.® 2.0 Protein Detection System for IHC work?
With two individually controlled sides, the system base allows for independent, vacuum-driven processing of either one or two IHC frames. Each of the IHC frames can process between 1 to 12 glass slides through independent vacuum ports.Each slide holder has an injection/recovery port that enables the manual addition, as well as the removal and recovery, of small volumes of antibodies or reagents; reagents can also be flushed using the vacuum feature if conservation is not a priority.
Comparable performance to traditional methods, even in archival tissue
The SNAP i.d.® 2.0 IHC System produces comparable staining to traditional protocols even in archival tissue. In the first example below, the system was used to detect Aquaporin 1 in archival human kidney tissue, with good results:
- Characteristic differential staining of kidney proximal tubule epithelium and renal corpuscle
- Robust, consistent staining despite processing 12 slides in parallel and shortening the handling time and protocol
- No blotchy artifacts that sometimes plague autostainers
- No apparent tissue degradation as compared with traditional protocols
- Classic histological stains such as hematoxylin can be applied using the same system
Detection of Aquaporin 1 in human kidney tissue (formalin-fixed and paraffin-embedded (FFPE): SNAP i.d.® 2.0 IHC System (sections 1–13) vs. Standard IHC protocol (section M1). Fifteen tissue sections (5 μm) were assembled on FisherBiotech® ProbeOn Plus™ slides. Slides were rehydrated and antigen retrieved (heat-induced epitope retrieval, HIER) using Reveal Decloaker (Biocare Medical, LLC) in a pressure cooker for 15 minutes at 110 °C. Thirteen slides were then processed using the SNAP i.d.® IHC system, and one was processed using the manual protocol. Blocking was performed by incubating 10 min with Punisher™ reagent (Biocare). After three washes with TBST, slides were incubated 2 h with Anti-Aquaporin 1 (Cat. No. AB2219, 1:2,000). After three more TBST washes, slides were incubated 10 min with Anti-Rabbit Secondary Antibody (Biocare). Signal was detected using a HRP-DAB detection kit (Biocare). Slides were washed three times with TBST and were counterstained with hematoxylin for 1 min. After three final washes, slides were dehydrated with four 5-minute changes of 100% ethanol, cleared with three changes of xylene and coverslipped with Ecomount medium (Biocare).