Millipore Sigma Vibrant Logo
Atención: Nos hemos mudado. Los productos Merck Millipore ya no pueden adquirirse en MerckMillipore.comMás información

480716 α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli

480716
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
480716-250MIU Ampolla de plást. 250 miu
Description
OverviewRecombinant, Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.
Note: 1 mU = 1 milliunit.
Catalogue Number480716
Brand Family Calbiochem®
SynonymsN-Acetylneuraminyl Hydrolase, Sialidase
References
ReferencesMeans, R.E. and Desrosiers, R.C. 2000. J. Virol. 74, 11181.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Product Information
CAS number9001-67-6
Activity≥5 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).
EC number3.2.1.18
FormLiquid
FormulationIn 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥40 units/mg protein
Physicochemical Information
ContaminantsN-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases: none detected.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSQQ3450000
Safety Information
R PhraseR: 20-36/37/38-42/43

Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli Certificados de análisis

CargoNúmero de lote
480716

Referencias bibliográficas

Visión general referencias
Means, R.E. and Desrosiers, R.C. 2000. J. Virol. 74, 11181.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-September-2007 RFH
SynonymsN-Acetylneuraminyl Hydrolase, Sialidase
DescriptionRecombinant, Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.
FormLiquid
FormulationIn 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.
Recommended reaction conditions50 mM sodium phosphate buffer, pH 6.0.
CAS number9001-67-6
RTECSQQ3450000
EC number3.2.1.18
ContaminantsN-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases: none detected.
Specific activity≥40 units/mg protein
Activity≥5 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Harmful
ReferencesMeans, R.E. and Desrosiers, R.C. 2000. J. Virol. 74, 11181.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.