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218725 Caspase-5, Human, Recombinant, E. coli

218725
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
218725-3000U Ampolla de plást. 3000 u
Description
OverviewRecombinant, human caspase-5 expressed in E. coli. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000.
M.W. 10,000 and 22,000 (heterodimer).
Catalogue Number218725
Brand Family Calbiochem®
SynonymsICErel-III, TY
References
ReferencesSchwartz, S. et al. 1999. Cancer Res. 59, 2995.
Fucheu, C., et al. 1996. Eur. J. Biochem. 236, 207.
Munday, N.A., et al. 1995. J. Biol. Chem. 270, 15870.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-LEHD-<i>p</i>NA (<a href="http://www.emdbiosciences.com/products/EMD_BIO-218805">Cat. No. 218805</a>) per min at 25°C, pH 7.4.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Purity≥80% by SDS-PAGE
Specific Activity≥100,000 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsAfter initial defrost, aliquot and freeze at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Caspase-5, Human, Recombinant, E. coli Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Caspase-5, Human, Recombinant, E. coli Certificados de análisis

CargoNúmero de lote
218725

Referencias bibliográficas

Visión general referencias
Schwartz, S. et al. 1999. Cancer Res. 59, 2995.
Fucheu, C., et al. 1996. Eur. J. Biochem. 236, 207.
Munday, N.A., et al. 1995. J. Biol. Chem. 270, 15870.

Folleto

Cargo
Caspases and other Apoptosis Related Tools Brochure
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-June-2008 RFH
SynonymsICErel-III, TY
DescriptionRecombinant, human caspase-5 expressed in E. coli. A member of the caspase-1 subfamily of cysteine proteases involved in Fas-mediated apoptosis. Caspase-5 cleavage site is reported to be Asp311 and Asp330. May also cleave at Asp121. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
Purity≥80% by SDS-PAGE
Specific activity≥100,000 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-LEHD-pNA (Cat. No. 218805) per min at 25°C, pH 7.4.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsAfter initial defrost, aliquot and freeze at -70°C.
Toxicity Standard Handling
ReferencesSchwartz, S. et al. 1999. Cancer Res. 59, 2995.
Fucheu, C., et al. 1996. Eur. J. Biochem. 236, 207.
Munday, N.A., et al. 1995. J. Biol. Chem. 270, 15870.