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17-701 EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

12 assays  RIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
Purchase on Sigma-Aldrich


Replacement Information

Tabla espec. clave

Key Applications
Catalogue Number17-701
Brand Family Upstate
Trade Name
  • EZ-Magna RIP
  • Upstate
DescriptionEZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit
OverviewRNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).

Features & Benefits:
-Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
-RNAse inhibitors and RNAse-free reagents
-Positive and negative controls
Alternate Names
  • Magnetic RIP kit
Materials Required but Not DeliveredMagna Grip™ Rack 8 well ( 20-400) (Now Available!) or similar magnetic rack.
Background InformationGene regulation plays a critical role in complex cellular processes such as development, differentiation, and cellular response to environmental changes. In addition to transcriptional regulation of gene expression by transcription factors, cells utilize post-transcriptional regulatory mechanisms. One such mechanism involves use of certain RNA-binding proteins (RBPs) to temporally and coordinately regulate the rate of mRNA translation of functionally related gene
products. While the regulation of gene expression by transcription factors has been well studied over time, the post-transcriptional regulation of mRNAs by RBPs and the role of non-coding RNAs in this process is a relatively nascent field that remains to be thoroughly explored.
Product Information
  • Magnetic Beads Protein A/G
  • RIP Wash Buffer
  • RIP Lysis Buffer
  • 0.5 M EDTA
  • 10% SDS
  • Salt Solution I
  • Salt Solution II
  • Precipitate Enhancer
  • Normal Mouse IgG
  • Rabbit IgG Purified
  • Protease Inhibitor Cocktail 200X
  • RNase Inhibitor
  • Proteinase K (10 mg/mL)
  • Nuclease free water
  • Positive Control Antibody (Anti-SNRNP70)
  • Control Primers
PresentationThree boxes containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions.
Includes positive control antibody and control primers.
Quality LevelMQ100
ApplicationRNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads. Control primers included.
Key Applications
  • RNA Binding Protein Immunoprecipitation (RIP)
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
Packaging Information
Material Size12 assays
Material PackageRIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
Transport Information
Supplemental Information
Global Trade Item Number
Número de referencia GTIN
17-701 04053252000867


EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit Ficha datos de seguridad (MSDS)


Ficha técnica de seguridad del material (MSDS) 

EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit Certificados de análisis

CargoNúmero de lote
Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit - 3678915 3678915

Referencias bibliográficas

Visión general referenciasAplicación Pub Med ID
LncRNA TUG1 sponges miR-204-5p to promote osteoblast differentiation through upregulating Runx2 in aortic valve calcification.
Yu, C; Li, L; Xie, F; Guo, S; Liu, F; Dong, N; Wang, Y
Cardiovasc Res  114  168-179  2018

Mostrar resumen
29016735 29016735
Down-regulation of LncRNA CCAT1 enhances radiosensitivity via regulating miR-148b in breast cancer.
Lai, Y; Chen, Y; Lin, Y; Ye, L
Cell Biol Int  42  227-236  2018

Mostrar resumen
29024383 29024383
The long non-coding RNA ENST00000547547 reduces 5-fluorouracil resistance of colorectal cancer cells via competitive binding to microRNA-31.
Li, J; Li, X; Cen, C; Ai, X; Lin, C; Hu, G
Oncol Rep  39  217-226  2018

Mostrar resumen
29115526 29115526
LncRNA myocardial infarction-associated transcript (MIAT) contributed to cardiac hypertrophy by regulating TLR4 via miR-93.
Li, Y; Wang, J; Sun, L; Zhu, S
Eur J Pharmacol  818  508-517  2018

Mostrar resumen
29157986 29157986
Long non-coding RNA ZFAS1 sponges miR-484 to promote cell proliferation and invasion in colorectal cancer.
Xie, S; Ge, Q; Wang, X; Sun, X; Kang, Y
Cell Cycle  17  154-161  2018

Mostrar resumen
29179614 29179614
Nkx2-2as Suppression Contributes to the Pathogenesis of Sonic Hedgehog Medulloblastoma.
Zhang, Y; Wang, T; Wang, S; Xiong, Y; Zhang, R; Zhang, X; Zhao, J; Yang, AG; Wang, L; Jia, L
Cancer Res  78  962-973  2018

Mostrar resumen
29229597 29229597
High-content hydrogen water-induced downregulation of miR-136 alleviates non-alcoholic fatty liver disease by regulating Nrf2 via targeting MEG3.
Wang, X; Wang, J
Biol Chem  399  397-406  2018

Mostrar resumen
29261513 29261513
Dual regulation of HMGB1 by combined JNK1/2-ATF2 axis with miR-200 family in nonalcoholic steatohepatitis in mice.
Chen, X; Ling, Y; Wei, Y; Tang, J; Ren, Y; Zhang, B; Jiang, F; Li, H; Wang, R; Wen, W; Lv, G; Wu, M; Chen, L; Li, L; Wang, H
FASEB J  32  2722-2734  2018

Mostrar resumen
29298863 29298863
LncRNA GAS5 regulates ischemic stroke as a competing endogenous RNA for miR-137 to regulate the Notch1 signaling pathway.
Chen, F; Zhang, L; Wang, E; Zhang, C; Li, X
Biochem Biophys Res Commun  496  184-190  2018

Mostrar resumen
29307821 29307821
Long non-coding RNA TCONS_00041960 enhances osteogenesis and inhibits adipogenesis of rat bone marrow mesenchymal stem cell by targeting miR-204-5p and miR-125a-3p.
Shang, G; Wang, Y; Xu, Y; Zhang, S; Sun, X; Guan, H; Zhao, X; Wang, Y; Li, Y; Zhao, G
J Cell Physiol  233  6041-6051  2018

Mostrar resumen
29319166 29319166


RNA-Binding Protein Immunoprecipitation

Licencias necesarias e Información técnica

White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

Manuales del usuario

Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

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Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits
Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits