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344146 FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli

344146
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Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
344146-100UG Ampolla de plást. 100 μg
Description
OverviewRecombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.
Catalogue Number344146
Brand Family Calbiochem®
SynonymsFTase, Farnesyl Proteintransferase
References
ReferencesZimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
Reiss, Y., et al. 1990. Cell 62, 81.
Product Information
FormLiquid
FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Quality LevelMQ100
Applications
ApplicationFPTase, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, is composed of an74 kDa α- and 47 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence.
Biological Information
Biological activity1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.
Purity≥90% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli Certificados de análisis

CargoNúmero de lote
344146

Referencias bibliográficas

Visión general referencias
Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
Reiss, Y., et al. 1990. Cell 62, 81.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-June-2008 RFH
SynonymsFTase, Farnesyl Proteintransferase
DescriptionRecombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion protein with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.
FormLiquid
FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Purity≥90% by SDS-PAGE
Biological activity1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesZimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
Reiss, Y., et al. 1990. Cell 62, 81.