Millipore Sigma Vibrant Logo
Atención: Nos hemos mudado. Los productos Merck Millipore ya no pueden adquirirse en MerckMillipore.comMás información

16-663 Magna ChIP™ Protein A+G Magnetic Beads

16-663
50 reactions  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Replacement Information

Ofertas especiales

Achieve first time ChIP success with EZ-ChIP Assays
Description
Catalogue Number16-663
Trade Name
  • Magna ChIP
DescriptionMagna ChIP™ Protein A+G Magnetic Beads
OverviewRecombinant protein A plus recombinant protein G covalently bound to magnetic beads. This magnetic A+G blend allows for the use of a wider range of antibodies than A or G alone and provides a rapid, reproducible and efficient reagent for collecting immunocomplexes for chromatin immunoprecipitation (ChIP) assays as well as RNA immunoprecipitation (RIP) assays. Compared with conventional agarose beads, magnetic beads significantly reduce handling times and mechanical stress on target immunocomplexes.
Alternate Names
  • ChIP magnetic beads
  • ChIP magnetic A/G beads
  • ChIP magnetic A+G beads
Background InformationChromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. Experiments comparing protein A vs. protein G vs. protein A/G magnetic bead blends revealed that a mixture of protein A and G beads worked well with a wide variety of antibody isotypes. The use of Protein A/G bead blends eliminated the need to consider which beads or kit to use in order match a particular antibody/bead binding affinity combination. In addition to simplifying the procedure, comparing the use of either protein A or protein G alone, protein A/G magnetic bead blends improved signal-to-noise ratios without decreasing the recovery of input chromatin in ChIP assays.
References
Product Information
HS Code3822 19 90
PresentationLiquid suspension. Supplied as magnetic bead slurry in phosphate buffered saline, pH 7.4, containing 0.01% Tween®-20 and 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis blend of protein A+G magnetic beads allows for the use of a wider range of antibodies than A or G alone & provides a rapid, reproducible & efficient collection of immunocomplexes for chromatin immunoprecipitations (ChIP) and RNA immunoprecipitations (RIP) assays
Application NotesUse 20 µL of bead suspension per ChIP application. Includes sufficient reagents for 50 precipitation reactions. Disperse beads thoroughly before pipetting by rapid vortex.
Biological Information
Analytes Available
  • Protein A+G
Physicochemical Information
Dimensions
Particle Size~3 µm
Volume1 mL
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Chromatin immunoprecipitation (ChIP) using HeLa nuclear extracts and the Magna ChIP™ A Kit (Cat. #17-610).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of shipment. Do Not Freeze.
Storage Temperature2 to 8 °C (Do not freeze)
Packaging Information
Material Size50 reactions
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
16-663 04053252421549

Documentation

Magna ChIP™ Protein A+G Magnetic Beads Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Magna ChIP™ Protein A+G Magnetic Beads Certificados de análisis

CargoNúmero de lote
Magna ChIP Protein A+G Magnetic Beads - 2110578 2110578
Magna ChIP Protein A+G Magnetic Beads - 2147110 2147110
Magna ChIP Protein A+G Magnetic Beads - 1964533 1964533
Magna ChIP Protein A+G Magnetic Beads - 2000831 2000831
Magna ChIP Protein A+G Magnetic Beads - 2040919 2040919
Magna ChIP Protein A+G Magnetic Beads - 2061459 2061459
Magna ChIP Protein A+G Magnetic Beads - 2091774 2091774
Magna ChIP Protein A+G Magnetic Beads - 2192075 2192075
Magna ChIP Protein A+G Magnetic Beads - 2207245 2207245
Magna ChIP Protein A+G Magnetic Beads - 2246827 2246827

Referencias bibliográficas

Visión general referenciasPub Med ID
PGC-1β regulates mouse carnitine-acylcarnitine translocase through estrogen-related receptor α.
Mar Gacias,Albert Pérez-Martí,Magdalena Pujol-Vidal,Pedro F Marrero,Diego Haro,Joana Relat
Biochemical and biophysical research communications  423  2011

Mostrar resumen
22713466 22713466