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17-704 Magna RIP™ Quad RNA-Binding Protein Immunoprecipitation Kit

17-704
48 assays  RIP Kit capacity: 48 RNA-binding protein immunoprecipitation assays
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Key Applications
RIP
Description
Catalogue Number17-704
Brand Family Upstate
Trade Name
  • Magna RIP
  • Upstate
DescriptionMagna RIP™ Quad RNA-Binding Protein Immunoprecipitation Kit
OverviewRNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).

Features & Benefits:
-Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
-RNAse inhibitors and RNAse-free reagents
-Negative controls
Alternate Names
  • Magnetic RIP kit
Materials Required but Not DeliveredMagna Grip™ Rack 8 well ( 20-400) (Now Available!) or similar magnetic rack.
Background InformationGene regulation plays a critical role in complex cellular processes such as development, differentiation, and cellular response to environmental changes. In addition to transcriptional regulation of gene expression by transcription factors, cells utilize post-transcriptional regulatory mechanisms. One such mechanism involves use of certain RNA-binding proteins (RBPs) to temporally and coordinately regulate the rate of mRNA translation of functionally related gene
products. While the regulation of gene expression by transcription factors has been well studied over time, the post-transcriptional regulation of mRNAs by RBPs and the role of non-coding RNAs in this process is a relatively nascent field that remains to be thoroughly explored.
References
Product Information
Components
  • Magnetic Beads Protein A/G
  • RIP Wash Buffer
  • RIP Lysis Buffer
  • 0.5 M EDTA
  • 10% SDS
  • Salt Solution I
  • Salt Solution II
  • Precipitate Enhancer
  • Normal Mouse IgG
  • Rabbit IgG Purified
  • Protease Inhibitor Cocktail 200X
  • RNase Inhibitor
  • Proteinase K (10 mg/mL)
  • Nuclease free water
PresentationEight boxes containing all necessary reagents to perform 48 individual RNA-binding protein immunoprecipitation (RIP) reactions.
Quality LevelMQ100
Applications
ApplicationRNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 48 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads.
Key Applications
  • RNA Binding Protein Immunoprecipitation (RIP)
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
Packaging Information
Material Size48 assays
Material PackageRIP Kit capacity: 48 RNA-binding protein immunoprecipitation assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
17-704 04053252025426

Documentation

Magna RIP™ Quad RNA-Binding Protein Immunoprecipitation Kit Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Magna RIP™ Quad RNA-Binding Protein Immunoprecipitation Kit Certificados de análisis

CargoNúmero de lote
Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit - 3678915 3678915

Referencias bibliográficas

Visión general referenciasPub Med ID
MicroRNA-466l Upregulates IL-10 Expression in TLR-Triggered Macrophages by Antagonizing RNA-Binding Protein Tristetraprolin-Mediated IL-10 mRNA Degradation.
Ma, Feng, et al.
Journal of immunology (Baltimore, Md. : 1950), (2010)  2009

Mostrar resumen
20410487 20410487
Advances in RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray profiling.
Baroni, Timothy E, et al.
Methods Mol. Biol., 419: 93-108 (2008)  2008

Mostrar resumen
18369977 18369977
Ribonomic and short hairpin RNA gene silencing methods to explore functional gene programs associated with tumor growth arrest.
Baroni, Timothy E, et al.
Methods Mol. Biol., 383: 227-44 (2007)  2007

Mostrar resumen
18217689 18217689
RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts.
Keene, Jack D, et al.
Nat Protoc, 1: 302-7 (2006)  2005

Mostrar resumen
17406249 17406249
Gene expression analysis of messenger RNP complexes.
Penalva, Luiz O F, et al.
Methods Mol. Biol., 257: 125-34 (2004)  2004

Mostrar resumen
14770002 14770002
Ribonomics: identifying mRNA subsets in mRNP complexes using antibodies to RNA-binding proteins and genomic arrays.
Tenenbaum, Scott A, et al.
Methods, 26: 191-8 (2002)  2002

Mostrar resumen
12054896 12054896
Identifying mRNA subsets in messenger ribonucleoprotein complexes by using cDNA arrays.
Tenenbaum, S A, et al.
Proc. Natl. Acad. Sci. U.S.A., 97: 14085-90 (2000)  1999

Mostrar resumen
11121017 11121017

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Categorías

Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits
Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits