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17-700 Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

17-700
12 assays  RIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Key Applications
RIP
Description
Catalogue Number17-700
Brand Family Upstate
Trade Name
  • Magna RIP
  • Upstate
DescriptionMagna RIP™ RNA-Binding Protein Immunoprecipitation Kit
OverviewRNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).

Features & Benefits:
-Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
-RNAse inhibitors and RNAse-free reagents
-Negative controls
Alternate Names
  • Magnetic RIP kit
Materials Required but Not DeliveredMagna Grip™ Rack 8 well ( 20-400) (Now Available!) or similar magnetic rack.
Background InformationGene regulation plays a critical role in complex cellular processes such as development, differentiation, and cellular response to environmental changes. In addition to transcriptional regulation of gene expression by transcription factors, cells utilize post-transcriptional regulatory mechanisms. One such mechanism involves use of certain RNA-binding proteins (RBPs) to temporally and coordinately regulate the rate of mRNA translation of functionally related gene
products. While the regulation of gene expression by transcription factors has been well studied over time, the post-transcriptional regulation of mRNAs by RBPs and the role of non-coding RNAs in this process is a relatively nascent field that remains to be thoroughly explored.
References
Product Information
Components
  • Magnetic Beads Protein A/G
  • RIP Wash Buffer
  • RIP Lysis Buffer
  • 0.5 M EDTA
  • 10% SDS
  • Salt Solution I
  • Salt Solution II
  • Precipitate Enhancer
  • Normal Mouse IgG
  • Rabbit IgG Purified
  • Protease Inhibitor Cocktail 200X
  • RNase Inhibitor
  • Proteinase K (10 mg/mL)
  • Nuclease free water
PresentationTwo boxes containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions.
Quality LevelMQ100
Applications
ApplicationRNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads.
Key Applications
  • RNA Binding Protein Immunoprecipitation (RIP)
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
Packaging Information
Material Size12 assays
Material PackageRIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
17-700 04053252010880

Documentation

Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit Certificados de análisis

CargoNúmero de lote
Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit - 3678915 3678915

Referencias bibliográficas

Visión general referenciasAplicación Pub Med ID
LncRNA-PVT1 promotes pancreatic cancer cells proliferation and migration through acting as a molecular sponge to regulate miR-448.
Zhao, L; Kong, H; Sun, H; Chen, Z; Chen, B; Zhou, M
J Cell Physiol  233  4044-4055  2018

Mostrar resumen
28657147 28657147
LncRNA ZFAS1 promotes cell migration and invasion of fibroblast-like synoviocytes by suppression of miR-27a in rheumatoid arthritis.
Ye, Y; Gao, X; Yang, N
Hum Cell  31  14-21  2018

Mostrar resumen
28721682 28721682
Effects of long noncoding RNA SPRY4-IT1-mediated EZH2 on the invasion and migration of lung adenocarcinoma.
Wen, X; Han, XR; Wang, YJ; Fan, SH; Zhuang, J; Zhang, ZF; Shan, Q; Li, MQ; Hu, B; Sun, CH; Wu, Q; Tan, JH; Wu, DM; Lu, J; Zheng, YL
J Cell Biochem  119  1827-1840  2018

Mostrar resumen
28796375 28796375
MRPL33 and its splicing regulator hnRNPK are required for mitochondria function and implicated in tumor progression.
Liu, L; Luo, C; Luo, Y; Chen, L; Liu, Y; Wang, Y; Han, J; Zhang, Y; Wei, N; Xie, Z; Wu, W; Wu, G; Feng, Y
Oncogene  37  86-94  2018

Mostrar resumen
28869607 28869607
RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis.
Sutherland, JM; Sobinoff, AP; Fraser, BA; Redgrove, KA; Siddall, NA; Koopman, P; Hime, GR; McLaughlin, EA
J Cell Physiol  233  3262-3273  2018

Mostrar resumen
28884822 28884822
Long non-coding RNA MALAT1 promotes oral squamous cell carcinoma development via microRNA-125b/STAT3 axis.
Chang, SM; Hu, WW
J Cell Physiol  233  3384-3396  2018

Mostrar resumen
28926115 28926115
Linc00511 acts as a competing endogenous RNA to regulate VEGFA expression through sponging hsa-miR-29b-3p in pancreatic ductal adenocarcinoma.
Zhao, X; Liu, Y; Li, Z; Zheng, S; Wang, Z; Li, W; Bi, Z; Li, L; Jiang, Y; Luo, Y; Lin, Q; Fu, Z; Rufu, C
J Cell Mol Med  22  655-667  2018

Mostrar resumen
28984028 28984028
Functional Role of a Novel Long Noncoding RNA TTN-AS1 in Esophageal Squamous Cell Carcinoma Progression and Metastasis.
Lin, C; Zhang, S; Wang, Y; Wang, Y; Nice, E; Guo, C; Zhang, E; Yu, L; Li, M; Liu, C; Hu, L; Hao, J; Qi, W; Xu, H
Clin Cancer Res  24  486-498  2018

Mostrar resumen
29101304 29101304
Lnc-NTF3-5 promotes osteogenic differentiation of maxillary sinus membrane stem cells via sponging miR-93-3p.
Peng, W; Zhu, SX; Wang, J; Chen, LL; Weng, JQ; Chen, SL
Clin Implant Dent Relat Res  20  110-121  2018

Mostrar resumen
29106055 29106055
Long non-coding RNA GAS5 antagonizes the chemoresistance of pancreatic cancer cells through down-regulation of miR-181c-5p.
Gao, ZQ; Wang, JF; Chen, DH; Ma, XS; Yang, W; Zhe, T; Dang, XW
Biomed Pharmacother  97  809-817  2018

Mostrar resumen
29112934 29112934

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Categorías

Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits
Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits