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346101 Androgen Receptor, His•Tag®, Human, Recombinant, S. frugiperda

346101
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
346101-5000U Plastic ampoule 5000 u
Description
OverviewRecombinant, human androgen receptor (AR) fused at the N-terminus to a Flag-Tag™ sequence and at the C-terminus to His•Tag® sequence, expressed in S. frugiperda insect cells expressed using a baculovirus expression system, and purified by affinity chromatography. The activity of androgen is mediated by the binding of the intracellular androgen receptor which, when activated, translocates to the nucleus and modulates specific gene transcription by interaction with cis-acting hormone response elements in the target genes.
Catalogue Number346101
Brand Family Calbiochem®
SynonymsAR, Human, Recombinant, Dihydrotestosterone Receptor, Human, Recombinant
References
ReferencesChen, G., et al. 2005. Prostate 63, 395.
Estebanez-Perpina, E., et al. 2005, J. Biol. Chem. 280, 8060.
Gao, S., et al. 2005. Mol. Endocrinol. 19, 1792.
Hillmer, A.M., et al. 2005. Am. J. Hum. Genet. 77, 140.
Kinoshita, M., et al. 2005, Int. J. Urol. 12, 390.
Lim, J.T., et al. 2005. Proc. Natl. Acad. Sci. U.S.A. 102, 5156;
Marques, R.B., et al. 2005. Int. J. Cancer 117, 221;
Metzger, E., et al. 2005. Nature 437, 436;
Murthy, S., et al. 2005. Prostate 64, 362;
Chang, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5946.
Product Information
Activity1.0 unit is equivalent to 1 ng protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.
Unit of DefinitionOne unit is defined as 1 ng purified protein.
FormLiquid
FormulationIn 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 8.0.
Quality LevelMQ100
Applications
Biological Information
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Androgen Receptor, His•Tag®, Human, Recombinant, S. frugiperda SDS

Title

Safety Data Sheet (SDS) 

Androgen Receptor, His•Tag®, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
346101

References

Reference overview
Chen, G., et al. 2005. Prostate 63, 395.
Estebanez-Perpina, E., et al. 2005, J. Biol. Chem. 280, 8060.
Gao, S., et al. 2005. Mol. Endocrinol. 19, 1792.
Hillmer, A.M., et al. 2005. Am. J. Hum. Genet. 77, 140.
Kinoshita, M., et al. 2005, Int. J. Urol. 12, 390.
Lim, J.T., et al. 2005. Proc. Natl. Acad. Sci. U.S.A. 102, 5156;
Marques, R.B., et al. 2005. Int. J. Cancer 117, 221;
Metzger, E., et al. 2005. Nature 437, 436;
Murthy, S., et al. 2005. Prostate 64, 362;
Chang, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5946.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-November-2008 JSW
SynonymsAR, Human, Recombinant, Dihydrotestosterone Receptor, Human, Recombinant
DescriptionRecombinant, human androgen receptor (AR) fused at the N-terminus to a Flag-Tag™ sequence and at the C-terminus to His•Tag® sequence, expressed in S. frugiperda insect cells expressed using a baculovirus expression system, and purified by affinity chromatography. The activity of androgen is mediated by the binding of the intracellular androgen receptor which, when activated, translocates to the nucleus and modulates specific gene transcription by interaction with cis-acting hormone response elements in the target genes.
FormLiquid
FormulationIn 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Activity1.0 unit is equivalent to 1 ng protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.
Unit definitionOne unit is defined as 1 ng purified protein.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesChen, G., et al. 2005. Prostate 63, 395.
Estebanez-Perpina, E., et al. 2005, J. Biol. Chem. 280, 8060.
Gao, S., et al. 2005. Mol. Endocrinol. 19, 1792.
Hillmer, A.M., et al. 2005. Am. J. Hum. Genet. 77, 140.
Kinoshita, M., et al. 2005, Int. J. Urol. 12, 390.
Lim, J.T., et al. 2005. Proc. Natl. Acad. Sci. U.S.A. 102, 5156;
Marques, R.B., et al. 2005. Int. J. Cancer 117, 221;
Metzger, E., et al. 2005. Nature 437, 436;
Murthy, S., et al. 2005. Prostate 64, 362;
Chang, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5946.