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176908 Anthrax Protective Antigen, Bacillus anthracis, Nicked, Recombinant, B. anthracis - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: PA 63

176908
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
176908-100UG Plastic ampoule 100 μg
Description
OverviewRecombinant, Bacillus anthracis anthrax protective antigen (nicked) expressed in a specialized strain of B. anthracis. PA63 is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA63 oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (Kd ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.
Catalogue Number176908
Brand Family Calbiochem®
SynonymsPA 63
References
ReferencesMogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7045.
Nassi, S., et al. 2002. Biochemistry 41, 1445.
Singh, Y., et al. 1999. Infect. Immun. 67, 1853.
Leppla, S.H. 1988. Methods Enzymol. 165, 103.
Product Information
ATP CompetitiveN
FormLyophilized
FormulationLyophilized from 100 mM NaCL, 10 mM BIS-Tris propane, 1.25% trehalose, pH 8.5.
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetEF and/or LF
PuritySingle major band at 63 kD by SDS-PAGE
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C or- 70°C) for long term storage or refrigerate (4°C) for short-term (few hours) storage. Avoid rapid freeze-thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anthrax Protective Antigen, Bacillus anthracis, Nicked, Recombinant, B. anthracis - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Anthrax Protective Antigen, Bacillus anthracis, Nicked, Recombinant, B. anthracis - Calbiochem Certificates of Analysis

TitleLot Number
176908

References

Reference overview
Mogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7045.
Nassi, S., et al. 2002. Biochemistry 41, 1445.
Singh, Y., et al. 1999. Infect. Immun. 67, 1853.
Leppla, S.H. 1988. Methods Enzymol. 165, 103.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-September-2009 RFH
SynonymsPA 63
DescriptionRecombinant, Bacillus anthracis anthrax protective antigen(nicked) expressed in a special strain of B. anthracis. PA63 is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA63 oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (Kd ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.
FormLyophilized
FormulationLyophilized from 100 mM NaCL, 10 mM BIS-Tris propane, 1.25% trehalose, pH 8.5.
PuritySingle major band at 63 kD by SDS-PAGE
SolubilityReconstitute in sterile 50% glycerol or sterile, distilled H₂O to a final concentration of 1 mg/ml. Addition of BSA to a concentration of 1 mg/ml may enhance stability.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C or- 70°C) for long term storage or refrigerate (4°C) for short-term (few hours) storage. Avoid rapid freeze-thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C.
Toxicity Standard Handling
ReferencesMogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7045.
Nassi, S., et al. 2002. Biochemistry 41, 1445.
Singh, Y., et al. 1999. Infect. Immun. 67, 1853.
Leppla, S.H. 1988. Methods Enzymol. 165, 103.