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371726 Anti-Giα-3- and Goα-Subunits, C-Terminal (345-354) Rabbit pAb

371726
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
MaRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
371726-50UL Glass bottle 50 ul
Description
OverviewRecognizes both Giα-3- and Goα-subunits. Does not cross-react with other G protein subunits.
Catalogue Number371726
Brand Family Calbiochem®
References
ReferencesRaymond, J.R., et al. 1993. Biochemistry 32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
Product Information
FormLiquid
FormulationSerum diluted in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Application CommentsThe Giα-3- and Goα-subunits share the last four amino acids (CGLY) of the immunizing peptide. Does not cross-react with other G-proteins. The specificity and cross-reactivity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, Giα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide (KNNLKECGLY) corresponding to amino acids 345-354 of mammalian Giα-3, conjugated to KLH
HostRabbit
IsotypeIgG
Species Reactivity
  • Mammals
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Giα-3- and Goα-Subunits, C-Terminal (345-354) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Giα-3- and Goα-Subunits, C-Terminal (345-354) Rabbit pAb Certificates of Analysis

TitleLot Number
371726

References

Reference overview
Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-November-2017 JSW
ApplicationImmunoblotting (1:1000)
DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes Giα-3 and Goα subunit proteins.
HostRabbit
Immunogena synthetic peptide (KNNLKECGLY) corresponding to amino acids 345-354 of mammalian Giα-3, conjugated to KLH
IsotypeIgG
Speciesmammalian
FormLiquid
FormulationSerum diluted in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
CommentsThe Giα-3- and Goα-subunits share the last four amino acids (CGLY) of the immunizing peptide. Does not cross-react with other G-proteins. The specificity and cross-reactivity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, Giα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesRaymond, J.R., et al. 1993. Biochemistry 32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.