Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

345860 Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10)

345860
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, RRMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
345860-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes GFAP in astrocytes induced by a variety of CNS injuries in human and rat brain tissues.
Catalogue Number345860
Brand Family Calbiochem®
SynonymsAnti-GFAP
Application Data
Detection of human glial fibrillary acidic protein by immunohistochemistry. Sample: Astrocytoma tissue. Primary antibody: Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) (Cat. No. 345860) (10 µg/ml). Detection: fluorescence.
References
ReferencesTohyama, T., et al. 1993. Am. J. Pathol. 142, 871.
Lee, V.M., et al. 1984. J. Neurochem. 42, 25.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlHuman or rat brain tissues
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoprecipitation
Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesELISA (0.1-0.5 µg/ml)
Immunoblotting (0.1-0.5 µg/ml)
Frozen Sections (10-50 µg/ml)
Immunoprecipitation (2-5 µg/ml)
Paraffin Sections (10-50 µg/ml, see comments)
Application CommentsProduct is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin's-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenbovine GFAP
ImmunogenBovine Brain
Clone2.2B10
HostRat
IsotypeIgG2a
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) SDS

Title

Safety Data Sheet (SDS) 

Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) Certificates of Analysis

TitleLot Number
345860

References

Reference overview
Tohyama, T., et al. 1993. Am. J. Pathol. 142, 871.
Lee, V.M., et al. 1984. J. Neurochem. 42, 25.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-August-2007 RFH
SynonymsAnti-GFAP
ApplicationELISA (0.1-0.5 µg/ml)
Immunoblotting (0.1-0.5 µg/ml)
Frozen Sections (10-50 µg/ml)
Immunoprecipitation (2-5 µg/ml)
Paraffin Sections (10-50 µg/ml, see comments)
Application Data
Detection of human glial fibrillary acidic protein by immunohistochemistry. Sample: Astrocytoma tissue. Primary antibody: Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) (Cat. No. 345860) (10 µg/ml). Detection: fluorescence.
DescriptionPurified rat monoclonal antibody. Recognizes the ~55 kDa glial fibrillary acidic protein (GFAP).
HostRat
Immunogen speciesBovine Brain
Immunogenbovine GFAP
Clone2.2B10
IsotypeIgG2a
Speciesbovine, human, rodent
Positive controlHuman or rat brain tissues
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsProduct is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin's-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesTohyama, T., et al. 1993. Am. J. Pathol. 142, 871.
Lee, V.M., et al. 1984. J. Neurochem. 42, 25.