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382158 Anti-Histone H3, Acetylated (1-20) Rabbit pAb

382158
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, TRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
382158-50UG Plastic ampoule 50 μg
Description
OverviewRecognizes the ~17 kDa acetylated histone H3 protein.
Catalogue Number382158
Brand Family Calbiochem®
Application Data
Detection of human acetylated histone H3 by immunoblotting. Samples: Acid-extract cells left untreated (lane 1) and sodium butyrate treated (lane 2). Primary antibody: Anti-Histone H3, Acetylated (1-20) Rabbit pAb (Cat. No. 382158) (0.01 µg/ml). Detection: chemiluminescence.
References
ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
Braunstein, M., et al. 1993. Genes Devel. 7, 592.
Perry, C.A., et al. 1993. Biochemistry 32, 13605.
Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Positive controlHeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesImmunoblotting (10-500 ng/ml, chemiluminescence)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (see comments)
Application CommentsFor immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Tetrahymena
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Histone H3, Acetylated (1-20) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Histone H3, Acetylated (1-20) Rabbit pAb Certificates of Analysis

TitleLot Number
382158

References

Reference overview
Braunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
Braunstein, M., et al. 1993. Genes Devel. 7, 592.
Perry, C.A., et al. 1993. Biochemistry 32, 13605.
Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-August-2007 RFH
ApplicationImmunoblotting (10-500 ng/ml, chemiluminescence)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (see comments)
Application Data
Detection of human acetylated histone H3 by immunoblotting. Samples: Acid-extract cells left untreated (lane 1) and sodium butyrate treated (lane 2). Primary antibody: Anti-Histone H3, Acetylated (1-20) Rabbit pAb (Cat. No. 382158) (0.01 µg/ml). Detection: chemiluminescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa acetylated histone H3 protein.
BackgroundThe acetylation of core histones regulates gene expression through alterations in the chromatin structure. Lysine acetylation in the amino-terminal domains of histones H3 and H4 can alter the topology of the DNA in chromatin and probably influence its higher-order folding.
HostRabbit
Immunogen speciesHuman
Immunogena diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH
IsotypeIgG
SpeciesTetrahymena, human
Positive controlHeLa cells
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsFor immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
Braunstein, M., et al. 1993. Genes Devel. 7, 592.
Perry, C.A., et al. 1993. Biochemistry 32, 13605.
Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.