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386040 Anti-Hsp90 Mouse mAb (AC88)

386040
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
386040-50UG Plastic ampoule 50 μg
Description
OverviewRecognizes the ~90 kDa Hsp90 protein.
Catalogue Number386040
Brand Family Calbiochem®
SynonymsAnti-Hsp82, Anti-Hsp85, Anti-Hsp89
Application Data
Detection of Hsp90 by immunoblotting. Samples: Native human Hsp90 protein (lane 1), recombinant human Hsp90β protein (lane 2), recombinant human Hsp90α protein (lane 3), whole cell lysate from heat shocked HeLa cells (lane 4), heat shocked 3T3 cells (lane 5), heat shocked PC-12 cells (lane 6), heat shocked CHO-K1 cells (lane 7), and heat shocked Rat-2 cells (lane 8). Primary antibody: Anti-Hsp90 Mouse mAb (AC88) (Cat. No. 386040) (1:1000). Detection: chemiluminescence.
References
ReferencesHarry, J.L. et al. 1990. Development 190, 305.
Minoo, P. et al. 1989 J. Cell. Biol. 109, 1937.
Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 50% glycerol, pH 7.2.
Positive controlHeat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml, colorimetric)
Immunoprecipitation (see comments)
Immunocytochemistry (see comments)
Application CommentsCross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Biological Information
ImmunogenHsp90 isolated from water mold, Achyla ambisexualis
ImmunogenAchyla ambisexualis
CloneAC88
HostMouse
IsotypeIgG₁
Species Reactivity
  • A Broad Range Of Species
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Hsp90 Mouse mAb (AC88) SDS

Title

Safety Data Sheet (SDS) 

Anti-Hsp90 Mouse mAb (AC88) Certificates of Analysis

TitleLot Number
386040

References

Reference overview
Harry, J.L. et al. 1990. Development 190, 305.
Minoo, P. et al. 1989 J. Cell. Biol. 109, 1937.
Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-August-2007 RFH
SynonymsAnti-Hsp82, Anti-Hsp85, Anti-Hsp89
ApplicationImmunoblotting (1 µg/ml, colorimetric)
Immunoprecipitation (see comments)
Immunocytochemistry (see comments)
Application Data
Detection of Hsp90 by immunoblotting. Samples: Native human Hsp90 protein (lane 1), recombinant human Hsp90β protein (lane 2), recombinant human Hsp90α protein (lane 3), whole cell lysate from heat shocked HeLa cells (lane 4), heat shocked 3T3 cells (lane 5), heat shocked PC-12 cells (lane 6), heat shocked CHO-K1 cells (lane 7), and heat shocked Rat-2 cells (lane 8). Primary antibody: Anti-Hsp90 Mouse mAb (AC88) (Cat. No. 386040) (1:1000). Detection: chemiluminescence.
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.
BackgroundHsp90 (heat shock protein 90) is a primarily cytoplasmic protein which exists in dimeric form. Hsp90 binds several cellular proteins such as retrovirus kinases (pp60v-src), steroid receptors, heme-regulated protein kinase, actin, and tubulin. Although Hsp90 expression is induced by stress, and thus may be an indicator of cell stress, its constitutive level may be up to 1-2% of the total cellular protein.
HostMouse
Immunogen speciesAchyla ambisexualis
ImmunogenHsp90 isolated from water mold, Achyla ambisexualis
CloneAC88
IsotypeIgG₁
Speciesa broad range of species
Positive controlHeat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
FormLiquid
FormulationIn 10 mM PBS, 50% glycerol, pH 7.2.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsCross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHarry, J.L. et al. 1990. Development 190, 305.
Minoo, P. et al. 1989 J. Cell. Biol. 109, 1937.
Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.