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407140 Anti-IP₃ Receptor Mouse mAb (IPR.1)

407140
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, H, M, Porcine, Rb, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
407140-50UG Plastic ampoule 50 μg
Description
OverviewRecognizes all of the ~260 kDa IP3 receptor subtypes.
Catalogue Number407140
Brand Family Calbiochem®
References
ReferencesBourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Positive controlLymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Cavallini, L., et al. 1996. J. Biol. Chem. 271, 5545. Immunoprecipitation Jho, D., et al. 2005. Circ. Res. 96, 1282. Immunohistochemistry Lagostena, L., et al. 2001. J. Physiol. 531, 693.
Key Applications Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Radioimmunoassay
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunoblotting (Western Blotting)
Application NotesELISA (1:1000)
Flow Cytometry (1:500)
Immunofluorescence (1:100)
Immunohistochemistry (1:100; see application references)
Immunoprecipitation (1:100; see application references)
Radioimmunoassay (1:5000)
Immunoblotting (see application references)
Application CommentsSpecific for the C-terminal cytoplasmic domain of the IP3 receptor. For immunohistochemistry, this antibody requires FITC and confocal microscopy. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide (GGVGDVLRKPS) corresponding to amino acids near the C-terminus of the IP₃ receptor
CloneIPR.1
HostMouse
IsotypeIgG2b
Species Reactivity
  • Bovine
  • Canine
  • Human
  • Mouse
  • Porcine
  • Rabbit
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-IP₃ Receptor Mouse mAb (IPR.1) SDS

Title

Safety Data Sheet (SDS) 

Anti-IP₃ Receptor Mouse mAb (IPR.1) Certificates of Analysis

TitleLot Number
407140

References

Reference overview
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-October-2008 JSW
ApplicationELISA (1:1000)
Flow Cytometry (1:500)
Immunofluorescence (1:100)
Immunohistochemistry (1:100; see application references)
Immunoprecipitation (1:100; see application references)
Radioimmunoassay (1:5000)
Immunoblotting (see application references)
DescriptionPurified mouse monoclonal antibody. Recognizes all of the ~260 kDa IP3 receptor subtypes.
BackgroundThe IP3 receptor is a homotetramer comprised of four ~260 kDa subunits. The IP3 receptor antibody induces significant amounts of internal Ca2+ release similar to IP3-mediated Ca2+ stimulation.
HostMouse
Immunogena synthetic peptide (GGVGDVLRKPS) corresponding to amino acids near the C-terminus of the IP₃ receptor
CloneIPR.1
IsotypeIgG2b
Speciesbovine, canine, human, mouse, porcine, rabbit, rat
Positive controlLymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsSpecific for the C-terminal cytoplasmic domain of the IP3 receptor. For immunohistochemistry, this antibody requires FITC and confocal microscopy. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesBourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
Application referencesImmunoblotting Cavallini, L., et al. 1996. J. Biol. Chem. 271, 5545. Immunoprecipitation Jho, D., et al. 2005. Circ. Res. 96, 1282. Immunohistochemistry Lagostena, L., et al. 2001. J. Physiol. 531, 693.