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PC05 Anti-c-Fos (Ab-2) (4-17) Rabbit pAb

PC05
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PC05-100UG Glass bottle 100 μg
Description
OverviewRecognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.
Catalogue NumberPC05
Brand Family Calbiochem®
References
ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
Rouscher III, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
Verma, I. 1986. Trends Genet. 2, 93.
Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive controlHepG2, NIH 3T3 cells, MCF-7 cells, colon or breast adenocarcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Not Free-floating Sections
Paraffin Sections
Application NotesFrozen sections (5-10 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/reaction)
Paraffin sections (5-10 µg/ml, pepsin or heat pre-treatment required)
Free-floating Sections (not recommended)
Application CommentsFor gel shift assays, use Cat. No. PC05L resuspended in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC05-100UG 07790788053932

Documentation

Anti-c-Fos (Ab-2) (4-17) Rabbit pAb Certificates of Analysis

TitleLot Number
PC05

References

Reference overview
DeTogni, P., et al. 1988. Cell Biol. 8, 2251.
Rouscher III, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
Verma, I. 1986. Trends Genet. 2, 93.
Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision17-September-2015 JSW
    ApplicationFrozen sections (5-10 µg/ml)
    Immunoblotting (1-5 µg/ml)
    Immunofluorescence (1-5 µg/ml)
    Immunoprecipitation (1-2 µg/reaction)
    Paraffin sections (5-10 µg/ml, pepsin or heat pre-treatment required)
    Free-floating Sections (not recommended)
    DescriptionProtein A purified rabbit polyclonal antibody. Recognizes v-fos and ~50-62 kDa c-fos proteins.
    BackgroundThe proto-oncogene fos has been implicated in cell growth, differentiation and development. Fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
    IsotypeIgG
    Specieshuman, mouse, rat
    Positive controlHepG2, NIH 3T3 cells, MCF-7 cells, colon or breast adenocarcinoma tissue
    FormLiquid
    FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsFor gel shift assays, use Cat. No. PC05L resuspended in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Highly Toxic
    ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
    Rouscher III, F.J., et al. 1988. Science 240, 1010.
    Sassone-Corsi, P., et al. 1988. Cell 54, 553.
    Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
    Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
    Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
    Verma, I. 1986. Trends Genet. 2, 93.
    Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.
    Citation
  • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.