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182515 Arp2/3 Complex Inhibitor I, CK-666 - CAS 442633-00-3 - Calbiochem

182515
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₁₈H₁₇FN₂O442633-00-3

Products

Catalogue NumberPackaging Qty/Pack
182515-25MG Glass bottle 25 mg
Description
OverviewA cell-permeable indolyl-fluorobenzamide compound that selectively inhibits actin assembly mediated by actin-related protein Arp2/3 complex of human, bovine, fission yeast S. pombe, and budding yeast S. cerevisiae origin (IC50 = 4, 17, 5, and 12 µM, respectively), without affecting S. pombe formin domain Cdc12(FH2)-mediated or the spontaneous actin polymerization. Shown to inhibit the actin filament "comet tails" formation around intracellular Listeria in infected SKOV3 cells (IC50 = 7 µM) in a reversible manner. Structrual studies indicate that CK-666 targets a pocket formed between subdomain 4 of Arp2 and subdomain 1 of Arp3, preventing Arp2/3 from shifting into an active conformation upon N-WASP-VCA binding. CK-666 and CK-869 (Cat. No. 182516) exhibit different modes of binding, resulting in their different yeast cross-reactivities. CK-689 (Cat. No. 182517) can serve as a negative control.
Catalogue Number182515
Brand Family Calbiochem®
SynonymsActin Assembly Inhibitor XIII, 2-Fluoro-N-(2-(2-Methyl-1H-indol-3-yl)ethyl)benzamide, CK-0944666
Application Data
References
ReferencesNolen, B.J., et al. 2009. Nature 460, 1031.
Product Information
CAS number442633-00-3
FormOff-white solid
Hill FormulaC₁₈H₁₇FN₂O
Chemical formulaC₁₈H₁₇FN₂O
Quality LevelMQ100
Applications
ApplicationArp2/3 Complex Inhibitor I, CK-666, CAS 442633-00-3, is a cell-permeable selective inhibitor of actin assembly mediated by actin-related protein Arp2/3 complex (IC50 = 4 uM in human).
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Regulatory Review
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Arp2/3 Complex Inhibitor I, CK-666 - CAS 442633-00-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Arp2/3 Complex Inhibitor I, CK-666 - CAS 442633-00-3 - Calbiochem Certificates of Analysis

TitleLot Number
182515

References

Reference overview
Nolen, B.J., et al. 2009. Nature 460, 1031.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-April-2011 RFH
SynonymsActin Assembly Inhibitor XIII, 2-Fluoro-N-(2-(2-Methyl-1H-indol-3-yl)ethyl)benzamide, CK-0944666
Application Data
DescriptionA cell-permeable indolyl-fluorobenzamide compound that selectively inhibits actin assembly mediated by actin-related protein Arp2/3 complex of human, bovine, fission yeast S. pombe, and budding yeast S. cerevisiae origin (IC50 = 4, 17, 5, and 12 µM, respectively), without affecting S. pombe formin domain Cdc12(FH2)-mediated or the spontaneous actin polymerization. Shown to inhibit the actin filament "comet tails" formation around intracellular Listeria in infected SKOV3 cells (IC50 = 7 µM) in a reversible manner. Structrual studies indicate that CK-666 targets a pocket formed between subdomain 4 of Arp2 and subdomain 1 of Arp3, preventing Arp2/3 from shifting into an active conformation upon N-WASP-VCA binding. CK-666 and CK-869 (Cat. No. 182516) exhibit different modes of binding, resulting in their different yeast cross-reactivities. CK-689 (Cat. No. 182517) can serve as a negative control.
FormOff-white solid
Intert gas (Yes/No) Packaged under inert gas
CAS number442633-00-3
Chemical formulaC₁₈H₁₇FN₂O
Purity≥95% by HPLC
SolubilityDMSO (50 mg/ml)
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Regulatory Review
ReferencesNolen, B.J., et al. 2009. Nature 460, 1031.