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219489 Ceramide Kinase Inhibitor, K1

219489
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₂₁H₂₈O₄1258005-85-4

Products

Catalogue NumberPackaging Qty/Pack
219489-1MG Plastic ampoule 1 mg
Description
Overview

This product has been discontinued.



A cell-permeable tetracyclic quinone compound that acts as a specific, reversible, and non-competitive inhibitor of CerK (ceramide kinase) activity (IC50 = ~5 µM) with little effect against SPHK1/2 or DGKγ (IC50 > 100 µM). Shown to reduce cellular C1P (ceramide-1-phosphate) synthesis by 40% at ≥ 20 µM in a rat basophilic leukemia cell line RBL-2H3 and block CerK-mediated degranulation in both RBL-2H3 and murine BMMC (bone marrow-derived mast cells) in a dose-dependent manner. Exhibits no cytotoxic effect against RBL-2H3 even at concentrations as high as 100 µM.

Catalogue Number219489
Brand Family Calbiochem®
SynonymsF-12509A Cyclic Product K1
References
ReferencesKim, J.W., et al. 2007. Biochim. Biophys. Acta 1771, 1262.
Kumada, H., et al. 2007. Biosci. Biotechnol. Biochem. 71, 2581
Kim, J.W., et al. 2005. Biochim. Biophys. Acta 1738, 82.
Product Information
CAS number1258005-85-4
DeclarationSold for research purposes only, pursuant to an agreement with Merck Japan.
FormYellow powder
Hill FormulaC₂₁H₂₈O₄
Chemical formulaC₂₁H₂₈O₄
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by NMR (mixture of enantiomers)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Ceramide Kinase Inhibitor, K1 SDS

Title

Safety Data Sheet (SDS) 

Ceramide Kinase Inhibitor, K1 Certificates of Analysis

TitleLot Number
219489

References

Reference overview
Kim, J.W., et al. 2007. Biochim. Biophys. Acta 1771, 1262.
Kumada, H., et al. 2007. Biosci. Biotechnol. Biochem. 71, 2581
Kim, J.W., et al. 2005. Biochim. Biophys. Acta 1738, 82.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-March-2011 RFH
SynonymsF-12509A Cyclic Product K1
DescriptionA cell-permeable tetracyclic quinone compound that acts as a specific, reversible, and non-competitive inhibitor of CerK (ceramide kinase) activity (IC50 ~5 µM) with little effect against SPHK1/2 or DGKγ (IC50 >100 µM). Shown to reduce cellular C1P (ceramide-1-phosphate) synthesis by 40% at ≥20 µM in a rat basophilic leukemia cell line RBL-2H3 and block CerK-mediated degranulation in both RBL-2H3 and murine BMMC (bone marrow-derived mast cells) in a dose-dependent manner. Exhibits no cytotoxic effect against RBL-2H3 even at concentrations as high as 100 µM.
FormYellow powder
Intert gas (Yes/No) Packaged under inert gas
CAS number1258005-85-4
Chemical formulaC₂₁H₂₈O₄
Structure formulaStructure formula
Purity≥95% by NMR (mixture of enantiomers)
SolubilityEthanol (20 mg/ml)
Storage -20°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesKim, J.W., et al. 2007. Biochim. Biophys. Acta 1771, 1262.
Kumada, H., et al. 2007. Biosci. Biotechnol. Biochem. 71, 2581
Kim, J.W., et al. 2005. Biochim. Biophys. Acta 1738, 82.