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220477 Chitosanase, Streptomyces sp. N174

220477
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
220477-10U Plastic ampoule 10 u
Description
OverviewNative Chitosanase from Streptomyces species. A member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethylcellulose. Acts by splitting the β1,4-glycosidic linkages of GlcNAc-GlcN (Km = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi.
Catalogue Number220477
Brand Family Calbiochem®
SynonymsChitosan N-acetylglucosaminohydrolase
References
ReferencesBoucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
Fukamizo, T., et al. 1995. Biochem J. 311, 377.
Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.
Product Information
CAS number51570-20-8
Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.
EC number3.2.1.132
FormLiquid
FormulationIn 50 M sodium acetate, 50% glycerol, pH 5.0.
PI7.5, temperature optimum 65°C for 10 minutes; recommended temperature 37°C
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥25 units/mg protein
Physicochemical Information
Contaminantschitinolytic activity: ≤0.01%, proteases: <0.25 Azocasein U/ml (One Azocasein unit is defined as the amount of protease that will liberate sufficient pigment to increase the optical density at 366 nm by 0.01 after incubation for 30 minutes with Azocasein)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Chitosanase, Streptomyces sp. N174 SDS

Title

Safety Data Sheet (SDS) 

Chitosanase, Streptomyces sp. N174 Certificates of Analysis

TitleLot Number
220477

References

Reference overview
Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
Fukamizo, T., et al. 1995. Biochem J. 311, 377.
Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-March-2019 JSW
SynonymsChitosan N-acetylglucosaminohydrolase
DescriptionNative Chitosanase from Streptomyces species. Chromatographically purified. Chitosanase is a member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethyl cellulose. Acts by splitting the b1,4-glycosidic linkages of GlcN-GlcN (Km = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi. Has an optimal pH of 5.5, an optimal temperature of 65°C for 10 min, and a recommended temperature of 37°C.
FormLiquid
FormulationIn 50 M sodium acetate, 50% glycerol, pH 5.0.
CAS number51570-20-8
EC number3.2.1.132
Contaminantschitinolytic activity: ≤0.01%, proteases: <0.25 Azocasein U/ml (One Azocasein unit is defined as the amount of protease that will liberate sufficient pigment to increase the optical density at 366 nm by 0.01 after incubation for 30 minutes with Azocasein)
Specific activity≥25 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBoucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
Fukamizo, T., et al. 1995. Biochem J. 311, 377.
Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.