Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on More

71009 CytoBuster™ Protein Extraction Reagent

Purchase on Sigma-Aldrich


Replacement Information


Catalogue NumberPackaging Qty/Pack
71009-3 Plastic ampoule 50 ml
71009-4 Glass bottle 250 ml
OverviewThe CytoBuster™ Protein Extraction Reagent is a proprietary formulation of detergents optimized for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of CytoBuster Protein Extraction Reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw. CytoBuster Protein Extraction Reagent has been specifically formulated for utilization in Western blotting, immunoprecipitation, and kinase/phosphatase assays. The reagent is compatible with protease inhibitors, kinase inhibitors, and phosphatase inhibitors.

Catalogue Number71009
Brand Family Novagen®
Product Information
Quality LevelMQ100
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
71009-3 04055977255690
71009-4 04055977255706


CytoBuster™ Protein Extraction Reagent SDS


Safety Data Sheet (SDS) 

CytoBuster™ Protein Extraction Reagent Certificates of Analysis

TitleLot Number


Protein Purification and Detection Tools
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression
Western Blotting Tools

Technical Info

Protein Blotting Handbook: 6th Edition


  • Kar Wah Leung, et al. (2008) Expression of ZnT and ZIP zinc transporters in the human RPE and their regulation by neurotrophic factors. Investigative Opthalmology and Visual Science 49, 1221-1231.
  • Chen-Hong Li, et al. (2008) Src tyrosine kinase alters gating of hyperpolarization-activated HCN4 pacemaker channel through Tyr531. American Journal of Physiology- Cell Physiology 294, C355-C362.
  • Yuk Cheung Chan and Po Sing Leung. (2007) Angiotensin II type 1 receptor-dependent nuclear factor-κB activation-mediated proinflammatory actions in a rat model of obstructive acute pancreatitis. Journal of Pharmacology and Experimental Therapeutics 323, 10-18.
  • Xiao-Yu Li, et al. (2007) APOBEC3G inhibits DNA strand transfer during HIV-1 reverse transcription. Journal of Biological Chemistry 282, 32065-32074.
  • Keyang Chen, et al. (2006) Alpha interferon potently enhances the anti-human immunodeficiency virus type 1 activity of APOBEC3G in resting primary CD4 T cells. Journal of Virology 80, 7645-7657.
  • Sun-Young Chang, et al. (2005) Heparan sulfate regulates the antiangiogenic activity of EMAP II at acidic pH. Molecular Pharmacology 67, 1534-1543.
  • Laurent Plantier, et al. (2005) Defect of hepatocyte growth factor production by fibroblasts in human pulmonary emphysema. American Journal of Physiology: Lung 288, L641-L647.
  • T. Michael Redmond, et al. (2005) Mutation of key residues of RPE65 abolishes its enzymatic role as isomerohydrolase in the visual cycle. Proceedings of the National Academy of Sciences (USA) 102, 13658-13663.
  • Dongqing Wang and S. Stoney Simons, Jr.. (2005) Corepressor binding to progesterone and glucocorticoid receptors involves the activation function-1 domain and is inhibited by molybdate. Molecular Endocrinology 19, 1483-1500.
  • Franziska Boess, et al. (2003) Gene expression in two hepatic cell lines, cultured primary hepatocytes and liver slices compared to the in vivo liver gene expression in rats: possible implications for toxicogenomics use of in vitro systems. Toxicological Science 73, 386-402.
  • Anne F. McGettrick, Ruth C. Barnes and D. Margaret Worrall. (2001) SCCA2 inhibits TNF-mediated apoptosis in transfected HeLa cells. European Journal of Biochemistry 268, 5868-5875.
  • User Protocols

    TB306 CytoBuster™ Protein Extraction Reagent