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259545 Deoxyhypusine Synthase Inhibitor, GC7 - CAS 150333-69-0 - Calbiochem

259545
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₈H₂₂N₄O₄S150333-69-0

Products

Catalogue NumberPackaging Qty/Pack
259545-10MG Glass bottle 10 mg
Description
OverviewA cell-permeable inhibitor that targets the spermidine-binding site of Deoxyhypusine Synthase (Ki = 9.7 nM), an enzyme which is responsible for the first step hypunisation of eukaryotic initiation factor 5A (eIF5A). In vivo, it demonstrates almost complete cessation of hypusine formation in CHO cells at ≥ 1 µM. Several studies indicate this compound as a useful tool for the down-regulation of eIF5A, a protein essential for cell proliferation. For example, it exhibits an anti-proliferative effect on both BCR-ABL -positive and -negative leukemia cell lines (K562 and HL-60) dose-dependently, at concentrations between 5 µM and 40 µM. In addition, it is shown to significantly impair the growth and invasion of primary HCC cells (HepG2 and Hep3B) in the 10-20 µM range, and against non-tumorigenic CL-48 cells at concentrations as high as 40 µM, with no apparent cytotoxicity. At 25 µM, GC7 treatment is shown to reverse eIF5a-mediated Skeletal Muscle Stem Cell Differentiation.
Catalogue Number259545
Brand Family Calbiochem®
SynonymsN1-guanyl-1,7-diamine-heptane, 1-(7-ammonioheptyl)guanidinium sulfate
References
ReferencesLee, N.P., et al. 2010. Int J Cancer. 4, 968.
Luchessi, A., et al. 2008. J Cell Physiol. 3, 480.
Balabanov, S., et al. 2007. Blood. 4, 1701.
Jakus, J., et al. 1993. J Biol Chem. 18, 13151.
Product Information
CAS number150333-69-0
FormWhite powder
Hill FormulaC₈H₂₂N₄O₄S
Chemical formulaC₈H₂₂N₄O₄S
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity>95% by NMR
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Deoxyhypusine Synthase Inhibitor, GC7 - CAS 150333-69-0 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Deoxyhypusine Synthase Inhibitor, GC7 - CAS 150333-69-0 - Calbiochem Certificates of Analysis

TitleLot Number
259545

References

Reference overview
Lee, N.P., et al. 2010. Int J Cancer. 4, 968.
Luchessi, A., et al. 2008. J Cell Physiol. 3, 480.
Balabanov, S., et al. 2007. Blood. 4, 1701.
Jakus, J., et al. 1993. J Biol Chem. 18, 13151.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-May-2012 JSW
SynonymsN1-guanyl-1,7-diamine-heptane, 1-(7-ammonioheptyl)guanidinium sulfate
DescriptionA cell-permeable inhibitor that targets the spermidine-binding site of Deoxyhypusine Synthase (Ki = 9.7 nM), an enzyme which is responsible for the first step hypunisation of eukaryotic initiation factor 5A (eIF5A). In vivo, it demonstrates almost complete cessation of hypusine formation in CHO cells at ≥ 1 µM. Several studies indicate this compound as a useful tool for the down-regulation of eIF5A, a protein essential for cell proliferation. For example, it exhibits an anti-proliferative effect on both BCR-ABL -positive and -negative leukemia cell lines (K562 and HL-60) dose-dependently, at concentrations between 5 µM and 40 µM. In addition, it is shown to significantly impair the growth and invasion of primary HCC cells (HepG2 and Hep3B) in the 10-20 µM range, and against non-tumorigenic CL-48 cells at concentrations as high as 40 µM, with no apparent cytotoxicity. At 25 µM, GC7 treatment is shown to reverse eIF5a-mediated Skeletal Muscle Stem Cell Differentiation.
FormWhite powder
CAS number150333-69-0
Chemical formulaC₈H₂₂N₄O₄S
Structure formulaStructure formula
Purity>95% by NMR
SolubilityH₂O (25 mg/ml; clear, colorless solution)
Storage -20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesLee, N.P., et al. 2010. Int J Cancer. 4, 968.
Luchessi, A., et al. 2008. J Cell Physiol. 3, 480.
Balabanov, S., et al. 2007. Blood. 4, 1701.
Jakus, J., et al. 1993. J Biol Chem. 18, 13151.