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371208 Dkk-1, Rat, Recombinant

371208
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Purity
≥95% by SDS-PAGE

Products

Catalogue NumberPackaging Qty/Pack
371208-10UG Glass bottle 10 μg
Description
OverviewRecombinant, rat Dickkopf related protein-1 (Dkk-1), consisting of amino acids 23-270, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. The balance between Wnt signaling and Dkk-1 inhibition is essential for bone formation and homeostasis.
Catalogue Number371208
Brand Family Calbiochem®
References
ReferencesLi, J. et al. 2006. Bone 36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
Niehrs, C. 2006. Oncogene 25, 7469.
Verani, R. et al. 2006. J. Neurochem. 101, 242.
Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
Adamska, M. et al. 2004. Dev. Biol. 272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B. et al. 2002. Nature 417, 664.
Mao, B. et al. 2001. Nature 411, 321.
Krupnik, V.E., et al. 1999. Gene 238, 301.
Product Information
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Quality LevelMQ100
Applications
Biological Information
Biological activityND₅₀ = 0.1-0.5 µg/ml as measured by inhibition of Wnt-3a-induced (10 ng/ml rmWnt-3a) alkaline phosphatase production by proliferation of MC3T3-E1 mouse osteoblastic cells.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Dkk-1, Rat, Recombinant SDS

Title

Safety Data Sheet (SDS) 

Dkk-1, Rat, Recombinant Certificates of Analysis

TitleLot Number
371208

References

Reference overview
Li, J. et al. 2006. Bone 36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
Niehrs, C. 2006. Oncogene 25, 7469.
Verani, R. et al. 2006. J. Neurochem. 101, 242.
Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
Adamska, M. et al. 2004. Dev. Biol. 272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B. et al. 2002. Nature 417, 664.
Mao, B. et al. 2001. Nature 411, 321.
Krupnik, V.E., et al. 1999. Gene 238, 301.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-June-2011 RFH
DescriptionRecombinant, rat Dickkopf related protein-1 (Dkk-1), consisting of amino acids 23-270, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. The balance between Wnt signaling and Dkk-1 inhibition is essential for bone formation and homeostasis.
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: ≤1.0 EU/µg protein
Biological activityND₅₀ = 0.1-0.5 µg/ml as measured by inhibition of Wnt-3a-induced (10 ng/ml rmWnt-3a) alkaline phosphatase production by proliferation of MC3T3-E1 mouse osteoblastic cells.
SolubilityReconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesLi, J. et al. 2006. Bone 36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
Niehrs, C. 2006. Oncogene 25, 7469.
Verani, R. et al. 2006. J. Neurochem. 101, 242.
Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
Adamska, M. et al. 2004. Dev. Biol. 272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B. et al. 2002. Nature 417, 664.
Mao, B. et al. 2001. Nature 411, 321.
Krupnik, V.E., et al. 1999. Gene 238, 301.