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328007 ERK Inhibitor II, FR180204 - CAS 865362-74-9 - Calbiochem

328007
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₁₈H₁₃N₇865362-74-9

Products

Catalogue NumberPackaging Qty/Pack
328007-1MG Plastic ampoule 1 mg
Description
OverviewA cell-permeable pyrazolopyridazinamine that acts as a potent, ATP-competitive inhibitor of ERK1 and ERK2 (IC50 = 510 nM and 330 nM; Ki = 310 nM and 140 nM, respectively). Exhibits ~20-fold greater selectivity over p38α (IC50 = 10 µM) and shows little activity towards IKKα, MEK1, MKK4, PDGFRα, PKCα, Src, and Syk even at concentrations as high as 30 µM. Shown to inhibit TGF-β−induced AP-1 activation in Mv1Lu epithelial cells (IC50 = 3.1 µM). ERK Inhibitor II, Negative Control is also available (Cat. No. 328008). Also available as a 10 mM solution in DMSO (Cat. No. 328010).
Catalogue Number328007
Brand Family Calbiochem®
Synonyms5-(2-Phenyl-pyrazolo[1,5-a]pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridazin-3-ylamine
References
ReferencesOhori, M., et al. 2005. Biochem. Biophys. Res. Commun. 336, 357.
Product Information
CAS number865362-74-9
ATP CompetitiveY
FormYellow solid
Hill FormulaC₁₈H₁₃N₇
Chemical formulaC₁₈H₁₃N₇
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetERK1 and ERK2
Primary Target IC<sub>50</sub>510 nM and 330 nM
Primary Target K<sub>i</sub>310 nM and 140 nM, against ERK1 and ERK2, respectively
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

ERK Inhibitor II, FR180204 - CAS 865362-74-9 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

ERK Inhibitor II, FR180204 - CAS 865362-74-9 - Calbiochem Certificates of Analysis

TitleLot Number
328007

References

Reference overview
Ohori, M., et al. 2005. Biochem. Biophys. Res. Commun. 336, 357.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-January-2012 RFH
Synonyms5-(2-Phenyl-pyrazolo[1,5-a]pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridazin-3-ylamine
DescriptionA cell-permeable pyrazolopyridazinamine that acts as a potent, ATP-competitive inhibitor of ERK1 and ERK2 (IC50 = 510 nM and 330 nM; Ki = 310 nM and 140 nM, respectively). Exhibits ~20-fold greater selectivity over p38α (IC50 = 10 µM) and shows little activity towards IKKα, MEK1, MKK4, PDGFRα, PKCα, Src, and Syk even at concentrations as high as 30 µM. Shown to inhibit TGF-β−induced AP-1 activation in Mv1Lu epithelial cells (IC50 of 3.1 µM). ERK Inhibitor II, Negative Control is also available (Cat. No. 328008). Also available in InSolution™ format (Cat. No. 328010).
FormYellow solid
Intert gas (Yes/No) Packaged under inert gas
CAS number865362-74-9
Chemical formulaC₁₈H₁₃N₇
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (10 mg/ml)
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesOhori, M., et al. 2005. Biochem. Biophys. Res. Commun. 336, 357.