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344045 FOCUS-Protease Arrest™ Cocktail - Calbiochem

A protease cocktail specifically developed for 2D gel sample preparation.

FOCUS-Protease Arrest™ Cocktail - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
344045
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
344045-1ML Plastic ampoule 1 ml
Description
OverviewA protease cocktail specifically developed for 2D gel sample preparation. Inhibits serine, cysteine, metalloproteinases, and calpain proteases from animal tissues, plants, yeast, and bacteria. FOCUS-Protease Arrest™ Cocktail at 1X concentration in extraction buffer, pH 7.0 - 8.0, inhibits 95-98% of protease activity.
Catalogue Number344045
Brand Family Calbiochem®
References
Product Information
CAS number67-68-5, 65391-42-6, 329-98-6, 103476-89-7, 110044-82-1, 9087-70-1, 30827-99-7
ATP CompetitiveN
FormLiquid
FormulationSupplied as 100X.
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetSerine, cysteine, metalloproteinases, and calpain proteases
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing dilution in extraction/lysis buffer or extract, the FOCUS-Protease Arrest™ inhibitors are stable for up to 2 weeks at 4°C or up to 4 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

FOCUS-Protease Arrest™ Cocktail - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

FOCUS-Protease Arrest™ Cocktail - Calbiochem Certificates of Analysis

TitleLot Number
344045
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-September-2007 RFH
DescriptionA protease inhibitor cocktail specifically developed for the inhibition of protease activity during protein purification and sample processing. Suitable for use with yeast, bacterial, plants, and mammalian tissues. No EDTA is used in this preparation. Compatible with FOCUS-Nuclease™ for removing nucleic acid contamination from samples. FOCUS-Protease-Arrest™ contains agents that inhibit serine, cysteine, metallo-proteases as well as calpains. FOCUS-Protease-Arrest™ contains both irreversible and reversible protease inhibitors. FOCUS-Protease Arrest™, used at 1:50 dilution in extraction buffer (pH 7-8) inhibits over 95-98% of protease activities.
FormLiquid
FormulationSupplied as 100X.
Recommended reaction conditions
1. Allow solution to warm to room temperature. The solution is in suspension form, vortex before pipetting. 2. Add FOCUS-Protease Arrest™ Cocktail at a volume of 10 µl/ml of extraction/lysis buffer or extract to achieve a 1X final concentration. Note: For greater protease inhibition, the Protease Arrest™ Cocktail may be added to extraction/lysis buffer at a volume of 13 µl/ml (1.3X final concentration).
CAS number67-68-5, 65391-42-6, 329-98-6, 103476-89-7, 110044-82-1, 9087-70-1, 30827-99-7
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing dilution in extraction/lysis buffer or extract, the FOCUS-Protease Arrest™ inhibitors are stable for up to 2 weeks at 4°C or up to 4 months at -20°C.
Toxicity Irritant