Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

368067 Granzyme B Substrate VIII, Colorimetric - Calbiochem

368067
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
368067-5MG Plastic ampoule 5 mg
Description
OverviewA colorimetric substrate for granzyme B (kcat/Km = 6.6 x 104 M-1s-1). Cleavage of pNA is monitored colorimetrically at ~405 nm (ε = 9,160 M-1cm-1).
Catalogue Number368067
Brand Family Calbiochem®
SynonymsAc-IEPD-pNA
References
ReferencesHarris, J.L., et al. 1998. J. Biol. Chem. 273, 27364.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
Product Information
ATP CompetitiveN
FormWhite lyophilized solid
Hill FormulaC₂₈H₃₈N₆O₁₁
Chemical formulaC₂₈H₃₈N₆O₁₁
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetColorimetric substrate for granzyme B
Primary Target IC<sub>50</sub>kcat/Km = 6.6 x 104 M⁻¹ sec⁻¹ as substrate for granzyme B
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Peptide SequenceAc-Ile-Glu-Pro-Asp-pNA
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Granzyme B Substrate VIII, Colorimetric - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Granzyme B Substrate VIII, Colorimetric - Calbiochem Certificates of Analysis

TitleLot Number
368067

References

Reference overview
Harris, J.L., et al. 1998. J. Biol. Chem. 273, 27364.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-July-2008 RFH
SynonymsAc-IEPD-pNA
DescriptionA colorimetric substrate for granzyme B (kcat/Km = 66,600 M-1s-1). Cleavage of pNA is monitored as an increase in absorbance at ~405 nm (ε = 9,160 M-1cm-1).
FormWhite lyophilized solid
Intert gas (Yes/No) Packaged under inert gas
Recommended reaction conditions0.25 µM substrate, 100 mM HEPES buffer, pH 7.5, and 10 mM DTT. Note: optimal enzyme concentration and incubation time should be determined empirically for each assay.
Chemical formulaC₂₈H₃₈N₆O₁₁
Peptide SequenceAc-Ile-Glu-Pro-Asp-pNA
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml)
Storage -20°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesHarris, J.L., et al. 1998. J. Biol. Chem. 273, 27364.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.