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375228 Hepatocyte Growth Factor, Human, Recombinant, S. frugiperda

375228
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Purity
≥95% by SDS-PAGE

Products

Catalogue NumberPackaging Qty/Pack
375228-5UG Plastic ampoule 5 μg
Description
OverviewRecombinant, human hepatocyte growth factor expressed in S. frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.
Catalogue Number375228
Brand Family Calbiochem®
SynonymsrhHGF
References
ReferencesKaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.
Product Information
FormLyophilized
FormulationLyophilized from a sterile-filtered solution in 20 mM Sodium Phosphate, 0.5 M NaCl, 50 µg BSA/µg HGF, pH 7.0.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 20-40 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in an HGF-responsive epithelial cell line, 4MBr-5
Purity≥95% by SDS-PAGE
Physicochemical Information
Contaminantsendotoxin: ≤1 EnU/µg HGF
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Hepatocyte Growth Factor, Human, Recombinant, S. frugiperda SDS

Title

Safety Data Sheet (SDS) 

Hepatocyte Growth Factor, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
375228

References

Reference overview
Kaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-November-2018 JSW
SynonymsrhHGF
DescriptionRecombinant, human hepatocyte growth factor expressed in Spodoptera frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.
FormLyophilized
FormulationLyophilized from a sterile-filtered solution in 20 mM Sodium Phosphate, 0.5 M NaCl, 50 µg BSA/µg HGF, pH 7.0.
Purity≥95% by SDS-PAGE
Contaminantsendotoxin: ≤1 EnU/µg HGF
Biological activityED₅₀ = 20-40 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in an HGF-responsive epithelial cell line, 4MBr-5
SolubilityReconstitute to a concentration of ≥1 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for up to 3 months at -70°C.
Toxicity Standard Handling
ReferencesKaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.