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400052 Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

400052
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
31282-04-9C₂₀H₃₇N₃O₁₃

Products

Catalogue NumberPackaging Qty/Pack
400052-20ML Alu drum 20 ml
400052-50ML Alu drum 50 ml
400052-5ML Alu drum 5 ml
Description
OverviewSterile liquid formulation of Cat. No. 400050.
Catalogue Number400052
Brand Family Calbiochem®
References
ReferencesSugimoto, K., et al. 1994. Plant J. 5, 863.
Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
Cullen, D., et al. 1987. Gene 57, 21.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
Santerre, R.F., et al. 1984. Gene 30, 147.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
Product Information
CAS number31282-04-9
FormLiquid
Formulation50 mg/ml active antibiotic in sterile-filtered PBS, pH 7.2.
Hill FormulaC₂₀H₃₇N₃O₁₃
Chemical formulaC₂₀H₃₇N₃O₁₃
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationHygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.
Biological Information
Biological activityPotency: ≥1000 U/mg
Purity≥85% by HPLC and TLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSWK2130000
Safety Information
R PhraseR: 26/27/28-37/38-41

Very toxic by inhalation, in contact with skin and if swallowed.
Irritating to respiratory system and skin.
Risk of serious damage to eyes.
S PhraseS: 23-26-36/37/39-45

Do not breathe fumes.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Highly Toxic & Carcinogenic / Teratogenic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem Certificates of Analysis

TitleLot Number
400052

References

Reference overview
Sugimoto, K., et al. 1994. Plant J. 5, 863.
Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
Cullen, D., et al. 1987. Gene 57, 21.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
Santerre, R.F., et al. 1984. Gene 30, 147.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.

Brochure

Title
Antibiotics Profiler
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-October-2007 RFH
DescriptionHygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA. Resistance to hygromycin B is conferred by a gene whose product, hygromycin B phospho-transferase that phosphorylates and inactivates the drug. Hygromycin B has been used to select mutants carrying the hygromycin B resistance gene in a wide variety of cells including bacterial, protozoal, yeast, fungal, plant, and mammalian cells. Typical concentrations used for mammalian cell selection are between 0.05 to 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.
FormLiquid
Formulation50 mg/ml active antibiotic in sterile-filtered PBS, pH 7.2.
CAS number31282-04-9
RTECSWK2130000
Chemical formulaC₂₀H₃₇N₃O₁₃
Structure formulaStructure formula
Purity≥85% by HPLC and TLC
Biological activityPotency: ≥1000 U/mg
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Toxicity Highly Toxic & Carcinogenic / Teratogenic
Merck USA index14, 4852
ReferencesSugimoto, K., et al. 1994. Plant J. 5, 863.
Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
Cullen, D., et al. 1987. Gene 57, 21.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
Santerre, R.F., et al. 1984. Gene 30, 147.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.