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407256 IGF-II, CF, Mouse, Recombinant

407256
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Purity
≥97% by SDS-PAGE

Products

Catalogue NumberPackaging Qty/Pack
407256-50UG Glass bottle 50 μg
Description
OverviewRecombinant, mouse insulin-like growth factor II (IGF-II) consisting of amino acids 25-91 and expressed in E. coli. Mouse IGF-II is synthesized as a 180 amino acid preproprecursor. Mature IGF-II is 91% and 97% amino acid identical to human and rat IGF-II, respectively.
Catalogue Number407256
Brand Family Calbiochem®
SynonymsMultiplication-stimulating polypeptide Somatomedin A
References
ReferencesOstrovsky, O., et al. 2009. Mol. Biol. Cell 20, 1855.
Chao, W. & P. A. DAmore, 2008. Cytokine Growth Factor Rev. 19, 111.
Belfiore, A., 2007. Curr. Pharm. Des. 13, 671.
Cornish, J., et al. 2007. Am. J. Physiol. Endocrinol. Metab. 292,E117.
Merrick, D., et al. 2007. BMC Dev. Biol. 7, 65.
Pavelic, J., et al. 2007. Indian J. Med. Res. 125, 511.
VarelaNieto, I., et al. 2007. Curr. Pharm. Des. 13,687.
Denley, A. et al. 2005. Cytokine Growth Factor Re,v. 16, 421.
LeRoith, D. & C.T. Roberts Jr., 2003. Cancer Lett. 195,127.
Buchanan, C.M., et al. 2001. Biochem. J. 360, 431.
Werner, H. & D. LeRoith, 2000. Cell. Mol. Life Sci. 57, 932.
Goya, L., et al. 1999. J. Biol. Chem. 274 24633.
Daughaday, W.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 5823.
Holthuizen, P.E., et al. 1993. Regul. Pept. 48, 77.
McCarthy, T.L., et al. 1992. Endocrinology 130, 1303.
Zindy, F., et al. 1992. J. Hepatol. 14, 30.
Rotwein, P. & L.J. Hall, 1990. DNA Cell Biol. 10,725.
Rotwein, P., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 265.
Stempien, M.M., et al. 1986. DNA 5, 357.
Product Information
FormSolid
FormulationLyophilized from 0.2 µm sterile-filtered Acetonitrile, TFA.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 2-10 ng/ml as measured in a serum-free cell proliferation assay using MCF-7 human breast adenocarcinoma cells.
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

IGF-II, CF, Mouse, Recombinant SDS

Title

Safety Data Sheet (SDS) 

IGF-II, CF, Mouse, Recombinant Certificates of Analysis

TitleLot Number
407256

References

Reference overview
Ostrovsky, O., et al. 2009. Mol. Biol. Cell 20, 1855.
Chao, W. & P. A. DAmore, 2008. Cytokine Growth Factor Rev. 19, 111.
Belfiore, A., 2007. Curr. Pharm. Des. 13, 671.
Cornish, J., et al. 2007. Am. J. Physiol. Endocrinol. Metab. 292,E117.
Merrick, D., et al. 2007. BMC Dev. Biol. 7, 65.
Pavelic, J., et al. 2007. Indian J. Med. Res. 125, 511.
VarelaNieto, I., et al. 2007. Curr. Pharm. Des. 13,687.
Denley, A. et al. 2005. Cytokine Growth Factor Re,v. 16, 421.
LeRoith, D. & C.T. Roberts Jr., 2003. Cancer Lett. 195,127.
Buchanan, C.M., et al. 2001. Biochem. J. 360, 431.
Werner, H. & D. LeRoith, 2000. Cell. Mol. Life Sci. 57, 932.
Goya, L., et al. 1999. J. Biol. Chem. 274 24633.
Daughaday, W.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 5823.
Holthuizen, P.E., et al. 1993. Regul. Pept. 48, 77.
McCarthy, T.L., et al. 1992. Endocrinology 130, 1303.
Zindy, F., et al. 1992. J. Hepatol. 14, 30.
Rotwein, P. & L.J. Hall, 1990. DNA Cell Biol. 10,725.
Rotwein, P., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 265.
Stempien, M.M., et al. 1986. DNA 5, 357.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-Sepmtember-2011 RFH
SynonymsMultiplication-stimulating polypeptide Somatomedin A
DescriptionRecombinant, mouse insulin-like growth factor II (IGF-II) consisting of amino acids 25-91 and expressed in E. coli. Mouse IGF-II is synthesized as a 180 amino acid preproprecursor. Mature IGF-II is 91% and 97% amino acid identical to human and rat IGF-II, respectively.
FormSolid
FormulationLyophilized from 0.2 µm sterile-filtered Acetonitrile, TFA.
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU/µg protein
Biological activityED₅₀ = 2-10 ng/ml as measured in a serum-free cell proliferation assay using MCF-7 human breast adenocarcinoma cells.
SolubilityReconstitute to 100 µg/ml in sterile PBS.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesOstrovsky, O., et al. 2009. Mol. Biol. Cell 20, 1855.
Chao, W. & P. A. DAmore, 2008. Cytokine Growth Factor Rev. 19, 111.
Belfiore, A., 2007. Curr. Pharm. Des. 13, 671.
Cornish, J., et al. 2007. Am. J. Physiol. Endocrinol. Metab. 292,E117.
Merrick, D., et al. 2007. BMC Dev. Biol. 7, 65.
Pavelic, J., et al. 2007. Indian J. Med. Res. 125, 511.
VarelaNieto, I., et al. 2007. Curr. Pharm. Des. 13,687.
Denley, A. et al. 2005. Cytokine Growth Factor Re,v. 16, 421.
LeRoith, D. & C.T. Roberts Jr., 2003. Cancer Lett. 195,127.
Buchanan, C.M., et al. 2001. Biochem. J. 360, 431.
Werner, H. & D. LeRoith, 2000. Cell. Mol. Life Sci. 57, 932.
Goya, L., et al. 1999. J. Biol. Chem. 274 24633.
Daughaday, W.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 5823.
Holthuizen, P.E., et al. 1993. Regul. Pept. 48, 77.
McCarthy, T.L., et al. 1992. Endocrinology 130, 1303.
Zindy, F., et al. 1992. J. Hepatol. 14, 30.
Rotwein, P. & L.J. Hall, 1990. DNA Cell Biol. 10,725.
Rotwein, P., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 265.
Stempien, M.M., et al. 1986. DNA 5, 357.