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407254 IGFBP-3, CF, Mouse, Recombinant

407254
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Purity
≥95% by SDS-PAGE

Products

Catalogue NumberPackaging Qty/Pack
407254-25UG Glass bottle 25 μg
Description
OverviewRecombinant, mouse insulin-like growth factor binding protein 3 (IGFBP-3) consisting of amino acids 22-291 and expressed in NS0-derived murine myeloma cells with four substitutions: Arg250Gln, Gln259Arg, Ser260Gly, Arg271Pro. Mouse IGFBP-3 cDNA encodes a 291 amino acid residue precursor protein with a putative 27 aa residue signal peptide.
Catalogue Number407254
Brand Family Calbiochem®
References
ReferencesSchuller, A.G.P., et al. 1994. Mol. Cell. Endoc. 104, 57.
Product Information
FormSolid
FormulationLyophilized from 0.2 µm filtered Acetonitrile, TFA.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 0.125-0.5 µg/ml as measured by inhibition of the biological activity of IGF-I or IGF-II (30 ng/ml) on MCF-7 human breast adenocarcinoma cells.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

IGFBP-3, CF, Mouse, Recombinant SDS

Title

Safety Data Sheet (SDS) 

IGFBP-3, CF, Mouse, Recombinant Certificates of Analysis

TitleLot Number
407254

References

Reference overview
Schuller, A.G.P., et al. 1994. Mol. Cell. Endoc. 104, 57.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-September-2011 RFH
DescriptionRecombinant, mouse insulin-like growth factor binding protein 3 (IGFBP-3) consisting of amino acids 22-291 and expressed in NS0-derived murine myeloma cells with four substitutions: Arg250Gln, Gln259Arg, Ser260Gly, Arg271Pro. Mouse IGFBP-3 cDNA encodes a 291 amino acid residue precursor protein with a putative 27 aa residue signal peptide.
FormSolid
FormulationLyophilized from 0.2 µm filtered Acetonitrile, TFA.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU/µg protein
Biological activityED₅₀ = 0.125-0.5 µg/ml as measured by inhibition of the biological activity of IGF-I or IGF-II (30 ng/ml) on MCF-7 human breast adenocarcinoma cells.
SolubilityReconstitute to 100 µg/ml in sterile PBS.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesSchuller, A.G.P., et al. 1994. Mol. Cell. Endoc. 104, 57.