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402176 ImmunoPen™

402176
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
402176-1EA Glass bottle 1 ea
Description
OverviewDesigned to minimize waste of antibody in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. When applied in a circle around the specimen, forms a hydrophobic barrier, retaining antibody. Sufficient for 500-800 individual applications. Not available for sale in Japan.
Catalogue Number402176
Brand Family Calbiochem®
References
Product Information
DeclarationNot available for sale in Japan.
Applications
Application NotesFrozen Sections
Immunostaining Barrier
Paraffin Sections
Application CommentsTo start the ImmunoPen™ shake well and gently press the tip straight down several times until the fluid starts soaking the tip.

Paraffin Section Method:
Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen™. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen™ barrier after the digestion is completed.
Frozen Section Method:
Apply the ImmunoPen™ circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsKeep cap tightly closed when pen is not in use, and store horizontally. Lasts for ~500-800 applications.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

ImmunoPen™ SDS

Title

Safety Data Sheet (SDS) 

ImmunoPen™ Certificates of Analysis

TitleLot Number
402176

Citations

Title
  • Nürnberger, A., et al. 2004. J. Histochem. Cytochem. 52, 1647.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision06-August-2007 RFH
    ApplicationFrozen Sections
    Immunostaining Barrier
    Paraffin Sections
    DescriptionDesigned to minimize antibody waste in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. The pen contains liquid resistant to water, alcohol, and acetone. When applied in a circle around the specimen the liquid forms a hydrophobic barrier, retaining antibody and minimizing waste of valuable reagents. Multiple sections, separated by individ-ual circular barriers, can be applied to the same slide. The ImmunoPen™ can be used for immunostaining procedures employing the following methods: PAP (peroxidase-antiperoxidase), ABC, enzymatic, and frozen section. While the barrier is insoluble in alcohol and acetone, it can be removed by xylene after the staining procedure is complete.
    CommentsTo start the ImmunoPen™ shake well and gently press the tip straight down several times until the fluid starts soaking the tip.

    Paraffin Section Method:
    Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen™. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen™ barrier after the digestion is completed.
    Frozen Section Method:
    Apply the ImmunoPen™ circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.
    Storage +15°C to +30°C
    Do Not Freeze Ok to freeze
    Special InstructionsKeep cap tightly closed when pen is not in use, and store horizontally. Lasts for ~500-800 applications.
    Toxicity Standard Handling
    Citation
  • Nürnberger, A., et al. 2004. J. Histochem. Cytochem. 52, 1647.