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407617 Interleukin-1β, Mouse, Recombinant, E. coli

407617
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
407617-5UG Plastic ampoule 5 μg
Description
OverviewRecombinant, mouse interleukin-1β expressed in E. coli. Suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing.
Catalogue Number407617
Brand Family Calbiochem®
References
ReferencesChun, S.Y., et al. 1995. Endocrinology 136, 3120.
Weidmann, B., et al. 1994. Nature 370, 434.
Dinarello, C.A., and Wolff, S.M. 1993. N. Engl. J. Med. 328, 106.
Product Information
FormLyophilized
FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀= 5-10 pg/ml as measured in a cell proliferation assay
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg IL-1β
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSNM9740000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Interleukin-1β, Mouse, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Interleukin-1β, Mouse, Recombinant, E. coli Certificates of Analysis

TitleLot Number
407617

References

Reference overview
Chun, S.Y., et al. 1995. Endocrinology 136, 3120.
Weidmann, B., et al. 1994. Nature 370, 434.
Dinarello, C.A., and Wolff, S.M. 1993. N. Engl. J. Med. 328, 106.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-May-2008 RFH
DescriptionRecombinant, mouse interleukin-1β expressed in E. coli. Suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing. Interleukin-1 is a protein produced by stimulated macrophages and many other cell types. The following biological activities are attributed to IL-1: activation of T cells resulting in the production of IL-2 and expression of IL-2 receptors; initiation of Acute Phase Response by induction of hepatic protein synthesis; increased proteolysis of muscle proteins, amino acid mobilization, and caloric and oxygen utilization; induction of bone resorption; stimulation of fibroblast proliferation; induction of slow-wave sleep; fever production; induction of prostaglandin and leukotriene production; stimulation of thymocyte proliferation; induction of B cell proliferation and maturation and increased immunoglobulin synthesis; induction of collagenase and other proteinases in synovial fibroblasts and articular chrondrocytes, suggesting a role in pathological states such as arthritis; activation of neutrophils. 1 µg is equivalent to 1.1 x 106 WHO units.
FormLyophilized
FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.
RTECSNM9740000
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: ≤1.0 EU/µg IL-1β
Biological activityED₅₀= 5-10 pg/ml as measured in a cell proliferation assay
SolubilityReconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Toxicity Standard Handling
Merck USA index14, 4993
ReferencesChun, S.Y., et al. 1995. Endocrinology 136, 3120.
Weidmann, B., et al. 1994. Nature 370, 434.
Dinarello, C.A., and Wolff, S.M. 1993. N. Engl. J. Med. 328, 106.