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407700 Interleukin-10, Human, Recombinant, S. frugiperda

407700
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
407700-5UG Plastic ampoule 5 μg
Description
OverviewRecombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. Potent modulator of monocyte/macrophage function. Exhibits anti-inflammatory effects by suppressing macrophage proliferation. Blocks lipopolysaccharide induction of MMP-2 and MMP-9. Known to reduce neuronal degeneration following CNS injury by blocking glutamate-induced caspase-3 activation. Human IL-10 is also active on murine cell lines.
Catalogue Number407700
Brand Family Calbiochem®
SynonymsrhIL-1αrhIL-1βrhIL-10
References
ReferencesBachis, A., et al. 2001. J. Neurosci. 21, 3104.
Fortunato, S.J., et al. 2001. Obstet. Gynecol. 98, 284.
Fleming, S.D., et al. 1996. J. Immunol. 156, 1143.
Wang, C.Q., et al. 1996. J. Cell Physiol. 166, 305.
Niro, H., et al. 1994. Int. Immunol. 6, 661.
Moore, K., et al. 1993. Annu. Rev. Immunol. 11, 165.
Vieira, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 1172.
Product Information
FormLyophilized
FormulationCarrier-free.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 0.2-1 ng/ml as measured in a cell proliferation assay using a mouse mast cell line, MC/9.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <100 pg/μg IL-10
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze -70°C. Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Interleukin-10, Human, Recombinant, S. frugiperda SDS

Title

Safety Data Sheet (SDS) 

Interleukin-10, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
407700

References

Reference overview
Bachis, A., et al. 2001. J. Neurosci. 21, 3104.
Fortunato, S.J., et al. 2001. Obstet. Gynecol. 98, 284.
Fleming, S.D., et al. 1996. J. Immunol. 156, 1143.
Wang, C.Q., et al. 1996. J. Cell Physiol. 166, 305.
Niro, H., et al. 1994. Int. Immunol. 6, 661.
Moore, K., et al. 1993. Annu. Rev. Immunol. 11, 165.
Vieira, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 1172.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-July-2008 RFH
SynonymsrhIL-1αrhIL-1βrhIL-10
DescriptionRecombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. Potent modulator of monocyte/macrophage function. Exhibits anti-inflammatory effects by suppressing macrophage proliferation. Human IL-10 has cross-species reactivity and is active in mouse cell lines.
FormLyophilized
FormulationCarrier-free.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: <100 pg/μg IL-10
Biological activityED₅₀ = 0.2-1 ng/ml as measured in a cell proliferation assay using a mouse mast cell line, MC/9.
SolubilityReconstitute in sterile water or buffer to a final concentration of 0.1-1 mg/ml as stock solution. Further dilute in buffer such as PBS, 1% BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze -70°C. Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesBachis, A., et al. 2001. J. Neurosci. 21, 3104.
Fortunato, S.J., et al. 2001. Obstet. Gynecol. 98, 284.
Fleming, S.D., et al. 1996. J. Immunol. 156, 1143.
Wang, C.Q., et al. 1996. J. Cell Physiol. 166, 305.
Niro, H., et al. 1994. Int. Immunol. 6, 661.
Moore, K., et al. 1993. Annu. Rev. Immunol. 11, 165.
Vieira, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 1172.