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440319 Lumican, His•Tag®, CF, Mouse, Recombinant

440319
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Purity
≥95% by SDS-PAGE

Products

Catalogue NumberPackaging Qty/Pack
440319-50UG Glass bottle 50 μg
Description
OverviewRecombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.
Catalogue Number440319
Brand Family Calbiochem®
References
ReferencesD'Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun. 365, 266.
Nikitovic, D. et al. 2008. IUBMB Life 60, 818.
Nikitovic, D. et al. 2008. FEBS J. 275, 350.
Ishiwata, T. et al. 2007. Oncol. Rep. 18, 537.
Vuillermoz, B. et al. 2004. Exp. Cell Res. 296,294.
Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci. 44, 2422.
Jepsen, K.J. et al. 2002. J. Biol. Chem. 277, 35532.
Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci. 41,3365.
Blochberger, T.C. et al. 1992. J. Biol. Chem. 267, 347.
Chakravarti, S. et al. 1998. J. Cell Biol. 141, 1277.
Product Information
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS.
Quality LevelMQ100
Applications
Biological Information
Biological activityCell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 10⁴ BCE C/D-1b bovine cornea-derived endothelial cells.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Lumican, His•Tag®, CF, Mouse, Recombinant SDS

Title

Safety Data Sheet (SDS) 

Lumican, His•Tag®, CF, Mouse, Recombinant Certificates of Analysis

TitleLot Number
440319

References

Reference overview
D'Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun. 365, 266.
Nikitovic, D. et al. 2008. IUBMB Life 60, 818.
Nikitovic, D. et al. 2008. FEBS J. 275, 350.
Ishiwata, T. et al. 2007. Oncol. Rep. 18, 537.
Vuillermoz, B. et al. 2004. Exp. Cell Res. 296,294.
Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci. 44, 2422.
Jepsen, K.J. et al. 2002. J. Biol. Chem. 277, 35532.
Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci. 41,3365.
Blochberger, T.C. et al. 1992. J. Biol. Chem. 267, 347.
Chakravarti, S. et al. 1998. J. Cell Biol. 141, 1277.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-June-2011 RFH
DescriptionRecombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: ≤1.0 EU/µg protein
Biological activityCell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 10⁴ BCE C/D-1b bovine cornea-derived endothelial cells.
SolubilityReconstitute to 200 µg/ml with sterile PBS.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesD'Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun. 365, 266.
Nikitovic, D. et al. 2008. IUBMB Life 60, 818.
Nikitovic, D. et al. 2008. FEBS J. 275, 350.
Ishiwata, T. et al. 2007. Oncol. Rep. 18, 537.
Vuillermoz, B. et al. 2004. Exp. Cell Res. 296,294.
Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci. 44, 2422.
Jepsen, K.J. et al. 2002. J. Biol. Chem. 277, 35532.
Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci. 41,3365.
Blochberger, T.C. et al. 1992. J. Biol. Chem. 267, 347.
Chakravarti, S. et al. 1998. J. Cell Biol. 141, 1277.