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444235 MMP-13 Substrate, Fluorogenic - Calbiochem

444235
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
444235-1MG Plastic ampoule 1 mg
Description
OverviewA quenched fluorescence substrate for MMP-13 (collagenase-3; kcat/Km = 1.09 x 106 M-1s-1). Less efficient as a substrate for MMP-1 (fibroblast collagenase) or MMP-8 (neutrophil collagenase).
Catalogue Number444235
Brand Family Calbiochem®
SynonymsCollagenase-3 Substrate, Fluorogenic
References
ReferencesKnauper, V., et al. 1996. J. Biol. Chem. 271, 1544.
Product Information
ATP CompetitiveN
FormYellow lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₅₁H₆₅N₁₃O₁₅
Chemical formulaC₅₁H₆₅N₁₃O₁₅
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetA quenched fluorescence substrate for MMP-13 (collagenase-3)
Primary Target IC<sub>50</sub>kcat/Km = 1.09 x 106 M⁻¹ sec⁻¹ for MMP-13 (collagenase-3)
Purity≥98% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceMCA-Pro-Cha-Gly-Nva-His-Ala-Dpa-NH₂ [Cha = L-cyclohexylalanine; Dpa = 3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl; Nva = L-norvaline]
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

MMP-13 Substrate, Fluorogenic - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

MMP-13 Substrate, Fluorogenic - Calbiochem Certificates of Analysis

TitleLot Number
444235

References

Reference overview
Knauper, V., et al. 1996. J. Biol. Chem. 271, 1544.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-August-2008 RFH
SynonymsCollagenase-3 Substrate, Fluorogenic
DescriptionA quenched fluorescence substrate for MMP-13 (collagenase-3; kcat/Km = 1.09 x 106 M-1s-1; excitation maximum: ~325 nm; emission maximum: ~393 nm). Less efficient as a substrate for MMP-1 (fibroblast collagenase) or MMP-8 (neutrophil collagenase).
FormYellow lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Chemical formulaC₅₁H₆₅N₁₃O₁₅
Peptide SequenceMCA-Pro-Cha-Gly-Nva-His-Ala-Dpa-NH₂ [Cha = L-cyclohexylalanine; Dpa = 3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl; Nva = L-norvaline]
Purity≥98% by HPLC
SolubilityDMSO (5 mg/ml)
Storage -20°C
Protect from moisture
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesKnauper, V., et al. 1996. J. Biol. Chem. 271, 1544.