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444224 MMP-2/MMP-9 Substrate II - Calbiochem

444224
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
444224-5MG Plastic ampoule 5 mg
Description
OverviewAn exceptional thiopeptide that is used as a substrate for the rapid, sensitive, and continuous spectrophotometric assay of Type IV collagenases, MMP-2 (gelatinase A), and MMP-9 (gelatinase B). Also acts as a substrate for vertebrate collagenase. Cleavage is monitored at 324 nm.
Catalogue Number444224
Brand Family Calbiochem®
SynonymsAc-Pro-Leu-Gly-(2-mercapto-4-methylpentanoyl)-Leu-Gly-OEt, Gelatinase A/Gelatinase B Substrate II
References
ReferencesWeingarten, H., et al. 1985. Anal. Biochem. 147, 437.
Weingarten, H., et al. 1985. Biochemistry 24, 6730.
Product Information
ATP CompetitiveN
FormWhite solid
Hill FormulaC₃₁H₅₃N₅O₈S
Chemical formulaC₃₁H₅₃N₅O₈S
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetSubstrate for the rapid, sensitive, and continuous spectrophotometric assay of Type IV collagenases, MMP-2 (gelatinase A), and MMP-9 (gelatinase B).
Purity≥97% by HPLC
Physicochemical Information
Cell permeableN
Peptide SequenceAc-Pro-Leu-Gly-SCH[CH₂CH(CH₃)₂]-CO-Leu-Gly-OC₂H₅
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsUnstable in solution; reconstitute just prior to use.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

MMP-2/MMP-9 Substrate II - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

MMP-2/MMP-9 Substrate II - Calbiochem Certificates of Analysis

TitleLot Number
444224

References

Reference overview
Weingarten, H., et al. 1985. Anal. Biochem. 147, 437.
Weingarten, H., et al. 1985. Biochemistry 24, 6730.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-August-2008 RFH
SynonymsAc-Pro-Leu-Gly-(2-mercapto-4-methylpentanoyl)-Leu-Gly-OEt, Gelatinase A/Gelatinase B Substrate II
DescriptionAn exceptional thiopeptide that is used as a substrate for the rapid, sensitive, and continuous spectrophotometric assay for Type IV collagenases MMP-2 (gelatinase A) and MMP-9 (gelatinase B). Acts as a substrate for vertebrate collagenase. Cleavage is monitored at 324 nm.
FormWhite solid
Recommended reaction conditions50 mM HEPES buffer, pH 6.5-7.0, 10 mM CaCl2, 0.5-1 mM 4,4'-dithiodipyridine, 0.01-5 mM substrate, and desired amount of activated enzyme; incubate at 25-26° while continuously monitoring cleavage at 324 nm.
Chemical formulaC₃₁H₅₃N₅O₈S
Peptide SequenceAc-Pro-Leu-Gly-SCH[CH₂CH(CH₃)₂]-CO-Leu-Gly-OC₂H₅
Purity≥97% by HPLC
Solubility50% acetic acid (10 mg/ml) or H₂O (1 mg/ml)
Storage -20°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsUnstable in solution; reconstitute just prior to use.
Toxicity Standard Handling
ReferencesWeingarten, H., et al. 1985. Anal. Biochem. 147, 437.
Weingarten, H., et al. 1985. Biochemistry 24, 6730.