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475904 MOWIOL® 4-88 Reagent

475904
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
475904-100GM Glass bottle 100 gm
Description
OverviewUsed for mounting cells on slides for detection of cell-surface antigens by immunofluorescence. Useful as an anti-fading agent.
Catalogue Number475904
Brand Family Calbiochem®
References
ReferencesLongin, A., et al. 1993. J. Histochem. Cytochem. 41, 1833.
Beug, H., et al. 1979. Cell 18, 375.
Product Information
CAS number9002-89-5
FormYellow solid
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSTR8100000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 weeks at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

MOWIOL® 4-88 Reagent SDS

Title

Safety Data Sheet (SDS) 

MOWIOL® 4-88 Reagent Certificates of Analysis

TitleLot Number
475904

References

Reference overview
Longin, A., et al. 1993. J. Histochem. Cytochem. 41, 1833.
Beug, H., et al. 1979. Cell 18, 375.

Citations

Title
  • Marie Johansson, et al. (2007) Activation of endosomal dynein motors by stepwise assembly of Rab7–RILP–p150Glued, ORP1L, and the receptor ßlll spectrin. Journal of Cell Biology 176, 459-471.
  • Madhavi P. Maddugoda, et al. (2007) Myosin VI and vinculin cooperate during the morphogenesis of cadherin cell cell contacts in mammalian epithelial cells. Journal of Cell Biology 178, 529-540.
  • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
  • Ganna Panasyuk, et al. (2006) Nuclear export of S6K1 II is regulated by protein kinase CK2 phosphorylation at ser 17. Journal of Biological Chemistry 281, 31188-31201.
  • Maria V. Baez and Graciela L. Boccaccio. (2005) Mammalian SMAUG is a translational repressor that forms cytoplasmic foci similar to stress granules. Journal of Biological Chemistry 280, 43131-43140.
  • Micah M. Bhatti and William J. Sullivan, Jr.. (2005) Histone acetylase GCN5 enters the nucleus via importin- in protozoan parasite Toxoplasma gondii. Journal of Biological Chemistry 280, 5902-5908.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision11-August-2008 RFH
    DescriptionUsed for mounting cells on slides for detection of cell-surface antigens by immunofluorescence. Useful as an anti-fading agent.
    FormYellow solid
    CAS number9002-89-5
    RTECSTR8100000
    SolubilityAdd 2.4 g of MOWIOL® 4-88 to 6 g of glycerol and stir to mix. Add 6 ml of water and stir for several hours at room temperature. Add 12 ml of 0.2 M Tris (pH 8.5) and heat to 50°C for 10 min. with occasional mixing. After the MOWIOL® 4-88 dissolves, clarify the solution by centrifugation at 5000 x g for 15 min.
    Storage +15°C to +30°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 weeks at 4°C.
    Toxicity Standard Handling
    ReferencesLongin, A., et al. 1993. J. Histochem. Cytochem. 41, 1833.
    Beug, H., et al. 1979. Cell 18, 375.
    Citation
  • Marie Johansson, et al. (2007) Activation of endosomal dynein motors by stepwise assembly of Rab7–RILP–p150Glued, ORP1L, and the receptor ßlll spectrin. Journal of Cell Biology 176, 459-471.
  • Madhavi P. Maddugoda, et al. (2007) Myosin VI and vinculin cooperate during the morphogenesis of cadherin cell cell contacts in mammalian epithelial cells. Journal of Cell Biology 178, 529-540.
  • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
  • Ganna Panasyuk, et al. (2006) Nuclear export of S6K1 II is regulated by protein kinase CK2 phosphorylation at ser 17. Journal of Biological Chemistry 281, 31188-31201.
  • Maria V. Baez and Graciela L. Boccaccio. (2005) Mammalian SMAUG is a translational repressor that forms cytoplasmic foci similar to stress granules. Journal of Biological Chemistry 280, 43131-43140.
  • Micah M. Bhatti and William J. Sullivan, Jr.. (2005) Histone acetylase GCN5 enters the nucleus via importin- in protozoan parasite Toxoplasma gondii. Journal of Biological Chemistry 280, 5902-5908.