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475989 MTT - CAS 298-93-1 - Calbiochem

475989
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
298-93-1C₁₈H₁₆BrN₅S

Products

Catalogue NumberPackaging Qty/Pack
475989-10GM Plastic ampoule 10 gm
475989-1GM Glass bottle 1 gm
Description
OverviewMembrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.
Catalogue Number475989
Brand Family Calbiochem®
Synonyms3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
References
ReferencesLiu, Y., et al. 1997. J. Neurochem. 69, 581.
Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.
Product Information
CAS number298-93-1
ATP CompetitiveN
FormYellow powder
Hill FormulaC₁₈H₁₆BrN₅S
Chemical formulaC₁₈H₁₆BrN₅S
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetUsed as a direct indicator of cytotoxicity and apoptosis.
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
ContaminantsSulfated ash: ≤0.5%; heavy metals: ≤0.001%
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSXF8060000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful & Carcinogenic / Teratogenic
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

MTT - CAS 298-93-1 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

MTT - CAS 298-93-1 - Calbiochem Certificates of Analysis

TitleLot Number
475989

References

Reference overview
Liu, Y., et al. 1997. J. Neurochem. 69, 581.
Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-December-2019 JSW
Synonyms3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
DescriptionMembrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.
FormYellow powder
CAS number298-93-1
RTECSXF8060000
Chemical formulaC₁₈H₁₆BrN₅S
Structure formulaStructure formula
Purity≥98% by HPLC
ContaminantsSulfated ash: ≤0.5%; heavy metals: ≤0.001%
SolubilityH₂O (5 mg/ml)
Storage +15°C to +30°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.
Toxicity Harmful & Carcinogenic / Teratogenic
ReferencesLiu, Y., et al. 1997. J. Neurochem. 69, 581.
Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.