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17-10499 Magna MeRIP™ m6A Kit- Transcriptome-wide Profiling of N6-Methyladenosine

10 Assays  
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Key Spec Table

Key Applications
RNA Detection, Purification with magnetic beads, RIP
Catalogue Number17-10499
Trade Name
  • EZ-Magna RIP
DescriptionMagna MeRIP™ m6A Kit- Transcriptome-wide Profiling of N6-Methyladenosine
OverviewN6-methyladenosine (m6A) represents an abundant RNA modification that is conserved across many different species, ranging from plants, to yeast, to mammals. Similar to the 5-methylcytosine (5-mc) modification of DNA, m6A is a reversible chemical modification of RNA. m6A RNA modifications are an emerging topic in controlling cell fate transitions in mammalian embryonic stem cells. m6A is one of the most prevalent modifications on both mRNAs and noncoding RNAs in eukaryotes with an estimated 12,000 m6A sites in over 7,000 genes. The mark is deposited by a heterodimer of methyltransferase-like 3 and 14 (Mettl3 and Mettl14) and can be removed by the RNA demethylase enzymes FTO and ALKBH5. Obesity risk gene FTO encodes the first identified m6A demethylase. Mutations in FTO have been associated with increased risk for obesity and type II diabetes. Recently, impairment of the status of m6A regulation has lead to prolonged expression of Nanog in ES cells and the inability to exit self-renewal stages toward differentiation.

Methylated (m6A) RNA immunoprecipitation (MeRIP) is a method to monitor the status of m6A and map the location of m6A RNA modifications transcriptome-wide. The Magna MeRIP M6A Kit uses the MeRIP method to enable identification and transcriptome-wide profiling of m6A RNA modifications. In the MeRIP assay, RNA is chemically fragmented into 100 nucleotides or smaller fragments followed by magnetic immunoprecipitation with a monoclonal antibody toward m6A. After immunoprecipitation, isolated RNA fragments can be subjected with qRT-PCR or RNA sequencing (MeRIP-seq). The streamlined protocol is easy to process and can produce results with high SN ratios.

Features and Benefits

  • Complete collection of MeRIP valiidated reagents to streamline the MeRIP method.
  • High signal to noise ratios using high affinity m6A monoclonal antibody and A/G magnetic beads.
  • Stem cell validated.
Alternate Names
  • RNA Methylation Kit
  • MeRIP-Seq Kit
  • N6-Methyladenosine Kit
  • M6A Immunoprecipitation Kit
Product Information
  • RNA Fragmentation Buffer 10X (0.5 mL)
  • 0.5M EDTA (0.5 mL)
  • IP Buffer 5X (15 mL)
  • Magnetic Beads A/G Blend (315 µL)
  • Anti-N6-methyladenosine (m6A), clone 17-3-4-1 (100 µg)
  • RNase Inhibitor (90 µL)
  • N6-Methyladenosine, 5′-monophosphate sodium salt (m6A) (10 mg)
  • Normal Mouse IgG (125 µg)
  • MeRIP Primers Human EEF1A1 Positive (75 µL)
  • MeRIP Primers Human EEF1A1 Negative (75 µL)
Quality LevelMQ300
ApplicationThe Magna MeRIP™ m6A Kit streamlines the MeRIP (Methylated RNA Immunoprecipitation) method to enable identification and transcriptome-wide profiling of m6A (N6-methyladenosine) RNA methylation sites. Suitable for MeRIP-Seq and qRT-PCR of methylated RNA transcripts.
Key Applications
  • RNA Detection
  • Purification with magnetic beads
  • RNA Binding Protein Immunoprecipitation (RIP)
Application NotesThe Magna MeRIP M6A Kit provides sufficient reagents for 10 MeRIP reactions using 5 µg of mRNA or 5 MeRIP reactions when 300 µg of total RNA is used.

Please read through the entire protocol and carefully plan your work before starting. The MeRIP method requires multiple steps and can be done over a two-day period or over multiple days. There are several stopping points to allow the method to be carried out over multiple days. The approximate time required for each step and potential stopping points are provided in the tables below.
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels.
Kit components are stable for 6 months from date of shipment when stored as directed.
Packaging Information
Material Size10 Assays
Transport Information
Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
17-10499 04055977296556


Magna MeRIP™ m6A Kit- Transcriptome-wide Profiling of N6-Methyladenosine SDS


Safety Data Sheet (SDS) 


Reference overviewPub Med ID
RNA N6-methyladenosine methyltransferase-like 3 promotes liver cancer progression through YTHDF2-dependent posttranscriptional silencing of SOCS2.
Chen, M; Wei, L; Law, CT; Tsang, FH; Shen, J; Cheng, CL; Tsang, LH; Ho, DW; Chiu, DK; Lee, JM; Wong, CC; Ng, IO; Wong, CM
Hepatology  67  2254-2270  2018

Show Abstract
29171881 29171881
R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling.
Su, R; Dong, L; Li, C; Nachtergaele, S; Wunderlich, M; Qing, Y; Deng, X; Wang, Y; Weng, X; Hu, C; Yu, M; Skibbe, J; Dai, Q; Zou, D; Wu, T; Yu, K; Weng, H; Huang, H; Ferchen, K; Qin, X; Zhang, B; Qi, J; Sasaki, AT; Plas, DR; Bradner, JE; Wei, M; Marcucci, G; Jiang, X; Mulloy, JC; Jin, J; He, C; Chen, J
Cell  172  90-105.e23  2018

Show Abstract
29249359 29249359
METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and Promotes Leukemogenesis via mRNA m6A Modification.
Weng, H; Huang, H; Wu, H; Qin, X; Zhao, BS; Dong, L; Shi, H; Skibbe, J; Shen, C; Hu, C; Sheng, Y; Wang, Y; Wunderlich, M; Zhang, B; Dore, LC; Su, R; Deng, X; Ferchen, K; Li, C; Sun, M; Lu, Z; Jiang, X; Marcucci, G; Mulloy, JC; Yang, J; Qian, Z; Wei, M; He, C; Chen, J
Cell Stem Cell  22  191-205.e9  2018

Show Abstract
29290617 29290617
Recognition of RNA N6-methyladenosine by IGF2BP proteins enhances mRNA stability and translation.
Huang, H; Weng, H; Sun, W; Qin, X; Shi, H; Wu, H; Zhao, BS; Mesquita, A; Liu, C; Yuan, CL; Hu, YC; Hüttelmaier, S; Skibbe, JR; Su, R; Deng, X; Dong, L; Sun, M; Li, C; Nachtergaele, S; Wang, Y; Hu, C; Ferchen, K; Greis, KD; Jiang, X; Wei, M; Qu, L; Guan, JL; He, C; Yang, J; Chen, J
Nat Cell Biol  20  285-295  2018

Show Abstract
29476152 29476152
FTO Plays an Oncogenic Role in Acute Myeloid Leukemia as a N6-Methyladenosine RNA Demethylase.
Li, Z; Weng, H; Su, R; Weng, X; Zuo, Z; Li, C; Huang, H; Nachtergaele, S; Dong, L; Hu, C; Qin, X; Tang, L; Wang, Y; Hong, GM; Huang, H; Wang, X; Chen, P; Gurbuxani, S; Arnovitz, S; Li, Y; Li, S; Strong, J; Neilly, MB; Larson, RA; Jiang, X; Zhang, P; Jin, J; He, C; Chen, J
Cancer Cell  127-141  2017

Show Abstract
28017614 28017614
Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control.
Barbieri, I; Tzelepis, K; Pandolfini, L; Shi, J; Millán-Zambrano, G; Robson, SC; Aspris, D; Migliori, V; Bannister, AJ; Han, N; De Braekeleer, E; Ponstingl, H; Hendrick, A; Vakoc, CR; Vassiliou, GS; Kouzarides, T
Nature  552  126-131  2017

Show Abstract
29186125 29186125


m6A RNA Modifications of Reprogramming Genes in Mouse and Human Embryonic Stem Cell

User Guides

Magna MeRIP™ m6A Kit Transcriptome-wide Profiling of N6-Methyladenosine