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475855 Miracloth

475855
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
475855-1R Glass bottle 1 r
Description
OverviewQuick filtration material for gelatinous homogenates composed of rayon-polyester with an acrylic binder. Useful for protoplast isolation. One roll equals 18 in. x 50 ft.

Miracloth is an autoclavable, quick filtration material for gelatinous homogenates that is composed of rayon-polyester with an acrylic binder. It is ideal for filtration of cleared neutralized lysates during medium- and large-scale plasmid purification to prevent contamination with loosely adherent pellet material and is useful for protoplast isolation.

Typical pore size: 22-25 µm. Can be autoclaved.
Catalogue Number475855
Brand Family Calbiochem®
References
ReferencesHardy, G.E., St. J., and Sivasithamparam, K. 1991. Soil Biol. Biochem. 23, 25.
Hanau, R.M., et al. 1989. Exp. Mycol. 13, 33.
Walker, S.J., et al. 1984. Environ. Exp. Botany 24, 113.
Binder, A. et al. 1977. J. Biol. Chem. 253, 3094.
Product Information
FormCloth roll
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Miracloth SDS

Title

Safety Data Sheet (SDS) 

Miracloth Certificates of Analysis

TitleLot Number
475855

References

Reference overview
Hardy, G.E., St. J., and Sivasithamparam, K. 1991. Soil Biol. Biochem. 23, 25.
Hanau, R.M., et al. 1989. Exp. Mycol. 13, 33.
Walker, S.J., et al. 1984. Environ. Exp. Botany 24, 113.
Binder, A. et al. 1977. J. Biol. Chem. 253, 3094.

Citations

Title
  • Amparo Lima, et al. (2006) A redox-active FKBP-type immunophilin functions in accumulation of the photosystem II supercomplex in Arabidopsis thaliana. Procedings of the National Academy of Science 103, 12631-12636.
  • Yuki Nakamura, et al. (2005) A novel phosphatidylcholine-hydrolyzing phospholipase C induced by phosphate starvation in Arabidopsis. Journal of Biological Chemistry 280, 7469-7476.
  • Yuki Nakamura, et al. (2005) A novel phosphatidylcholine-hydrolyzing phospholipase C induced by phosphate starvation in Arabidopsis. Journal of Biological Chemistry 280, 7469-7476.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision15-May-2009 JSW
    DescriptionQuick filtration material for gelatinous homogenates and for protoplast isolation. Composed of rayon polyester with a pore size of 22-25 µm and an acrylic binder. One roll is 18 inches x 50 feet. Material can be autoclaved without compromising the integrity of the product. Although it is chemically resistant to some organic solvents, Miracloth will dissolve in high pH buffers (pH >9). Useful for protoplast isolation.
    FormCloth roll
    Storage +15°C to +30°C
    Do Not Freeze Ok to freeze
    Toxicity Standard Handling
    ReferencesHardy, G.E., St. J., and Sivasithamparam, K. 1991. Soil Biol. Biochem. 23, 25.
    Hanau, R.M., et al. 1989. Exp. Mycol. 13, 33.
    Walker, S.J., et al. 1984. Environ. Exp. Botany 24, 113.
    Binder, A. et al. 1977. J. Biol. Chem. 253, 3094.
    Citation
  • Amparo Lima, et al. (2006) A redox-active FKBP-type immunophilin functions in accumulation of the photosystem II supercomplex in Arabidopsis thaliana. Procedings of the National Academy of Science 103, 12631-12636.
  • Yuki Nakamura, et al. (2005) A novel phosphatidylcholine-hydrolyzing phospholipase C induced by phosphate starvation in Arabidopsis. Journal of Biological Chemistry 280, 7469-7476.
  • Yuki Nakamura, et al. (2005) A novel phosphatidylcholine-hydrolyzing phospholipase C induced by phosphate starvation in Arabidopsis. Journal of Biological Chemistry 280, 7469-7476.