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411483 Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate

411483
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
411483-200UG Plastic ampoule 200 μg
Description
Catalogue Number411483
Brand Family Calbiochem®
References
ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
Jefferis, R., et al. 1986. Immunol. Letters 10, 223.
Product Information
FormLiquid
FormulationIn PBS, 1% BSA, pH 7.4.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Application NotesELISA (1:500-1:2000, see comments)
Application CommentsBiotinylated with hydroxysuccinimide ester biotin. This biotin-labeled antibody is an extremely versatile reagent because of the high binding affinity for avidin and avidin-labeled compounds. Variables associated with assay conditions will dictate the proper working dilution.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: ≤0.1%
Human IgG2: ≤1%
Human IgG3: 100%
Human IgG4: ≤0.1%
Human IgA: ≤0.1%
Human IgE: ≤0.1%
Human IgM: ≤0.1%

Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: <0.001%
Human IgG2: <0.001%
Human IgG3: 100%
Human IgG4: <0.001%
Human IgA: <0.001%
Human IgE: <0.001%
Human IgM: <0.001%
Biological Information
ImmunogenHuman
CloneHP6047
HostMouse
IsotypeIgG₃
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate SDS

Title

Safety Data Sheet (SDS) 

Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate Certificates of Analysis

TitleLot Number
411483

References

Reference overview
Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
Jefferis, R., et al. 1986. Immunol. Letters 10, 223.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-February-2008 JSW
ApplicationELISA (1:500-1:2000, see comments)
DescriptionPurified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG3 hinge region.
HostMouse
Immunogen speciesHuman
CloneHP6047
IsotypeIgG₃
FormLiquid
FormulationIn PBS, 1% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsBiotinylated with hydroxysuccinimide ester biotin. This biotin-labeled antibody is an extremely versatile reagent because of the high binding affinity for avidin and avidin-labeled compounds. Variables associated with assay conditions will dictate the proper working dilution.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: ≤0.1%
Human IgG2: ≤1%
Human IgG3: 100%
Human IgG4: ≤0.1%
Human IgA: ≤0.1%
Human IgE: ≤0.1%
Human IgM: ≤0.1%

Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: <0.001%
Human IgG2: <0.001%
Human IgG3: 100%
Human IgG4: <0.001%
Human IgA: <0.001%
Human IgE: <0.001%
Human IgM: <0.001%
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
Jefferis, R., et al. 1986. Immunol. Letters 10, 223.