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480030 NEMO-Binding Domain Binding Peptide, Cell-Permeable, Negative Control - Calbiochem

480030
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₅₄H₂₄₉N₄₇O₄₂S

Products

Catalogue NumberPackaging Qty/Pack
480030-500UG Plastic ampoule 500 μg
Description
OverviewA cell-permeable Antennapedia-NBD mutated (Trp739 and Trp741 replaced with Ala) fusion peptide analog of NEMO-Binding Domain Binding Peptide (Cat. No. 480025) that serves as a negative control. Shown to be defective in binding to NEMO. Does not affect the TNF-α-induced phosphorylation of c-Jun and DNA binding of the transcription factor Oct-1.
Catalogue Number480030
Brand Family Calbiochem®
SynonymsDRQIKIWFQNRRMKWKK-TALDASALQTE, NBD-Binding Peptide, Cell-Permeable, Negative Control
References
ReferencesJimi, E., et al. 2004. Nat. Med., 10, 617.
May, M.J., et al. 2000. Science 289, 1550.
Product Information
ATP CompetitiveN
FormLyophilized off-white solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₁₅₄H₂₄₉N₄₇O₄₂S
Chemical formulaC₁₅₄H₂₄₉N₄₇O₄₂S
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetNegative control defective in binding to NEMO
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceH₂N-Asp-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Thr-Ala-Leu-Asp-Ala-Ser-Ala-Leu-Gln-Thr-Glu-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

NEMO-Binding Domain Binding Peptide, Cell-Permeable, Negative Control - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

NEMO-Binding Domain Binding Peptide, Cell-Permeable, Negative Control - Calbiochem Certificates of Analysis

TitleLot Number
480030

References

Reference overview
Jimi, E., et al. 2004. Nat. Med., 10, 617.
May, M.J., et al. 2000. Science 289, 1550.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-August-2008 RFH
SynonymsDRQIKIWFQNRRMKWKK-TALDASALQTE, NBD-Binding Peptide, Cell-Permeable, Negative Control
DescriptionA cell-permeable, Antennapedia-NBD mutated (Trp739 → Ala and Trp741 → Ala) fusion peptide analog of NEMO-Binding Domain Binding peptide (Cat. No. 480025) that serves as a negative control. Reported to be defective in binding to NEMO. Does not affect the TNF-α-induced phosphorylation of c-Jun or DNA binding of the transcription factor Oct-1.
FormLyophilized off-white solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₅₄H₂₄₉N₄₇O₄₂S
Peptide SequenceH₂N-Asp-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Thr-Ala-Leu-Asp-Ala-Ser-Ala-Leu-Gln-Thr-Glu-OH
Purity≥95% by HPLC
SolubilityDMSO (50 mg/ml). Note: This peptide will precipitate when added to tissue culture medium even at 100 µM concentrations; however, sufficient material will stay in solution to permeate cells.
Storage -20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesJimi, E., et al. 2004. Nat. Med., 10, 617.
May, M.J., et al. 2000. Science 289, 1550.