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480065 Necrostatin-1 - CAS 4311-88-0 - Calbiochem

480065
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
4311-88-0C₁₃H₁₃N₃OS

Products

Catalogue NumberPackaging Qty/Pack
480065-5MG Plastic ampoule 5 mg
Description
OverviewA cell-permeable, potent, and selective blocker of necroptosis (EC50 = 494 nM in FADD-deficient Jurkat cells treated with TNF-α), a nonapoptotic necrotic cell death pathway mediated by death-domain receptors (DRs) that offers neuroprotection in a murine model of ischemic brain injury. Exhibits no effect on DR-induced apoptosis. Also acts as a selective and ATP-competitive inhibitor of RIP1 kinase with negligible effect of RIP2 kinase activity. Nec-1 target appears to be a critical common necroptotic step upstream of execution events and downstream of DRs. Inactive control, Nec-1i, is also available (Cat. No. 480066). Also available as a 25 mM solution in DMSO (Cat. No. 505224).
Catalogue Number480065
Brand Family Calbiochem®
SynonymsNecrosome Inhibitor I, 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, Nec-1, Methyl-thiohydantoin-tryptophan, MTH-Trp, Receptor-Interacting Protein 1 Inhibitor I, RIPK 1 Inhibitor I, Nec1, RIP1 Inhibitor I, Necrosis Inhibitor II
References
ReferencesDegterev, A., et al. 2013. Nat. Chem. Biol. 9, 192.
Degterev, A., et al. 2012. Cell Death Differ. 20, 366.
Christofferson, D.E., et al. 2012. Cell Death Dis. 3, e320.
Takahashi, N., et al. 2012. Cell Death Dis. 3, e437.
Degterev, A., et al. 2008. Nat. Chem. Biol. 4, 313.
Degterev, A., et al. 2005. Nat. Chem. Biol. 1, 112.
Muller, A.J., et al. 2005. Nat. Med. 11, 312.
Teng, X., et al. 2005. Bioorg. Med. Chem. Lett. 15, 5039.
Product Information
CAS number4311-88-0
ATP CompetitiveY
FormYellow crystalline solid
Hill FormulaC₁₃H₁₃N₃OS
Chemical formulaC₁₃H₁₃N₃OS
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetBlocker of necroptosis
Primary Target IC<sub>50</sub>EC50 = 494 nM in blocking necrosis in FADD-deficient Jurkat cells treated with TNF-α
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Necrostatin-1 - CAS 4311-88-0 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Necrostatin-1 - CAS 4311-88-0 - Calbiochem Certificates of Analysis

TitleLot Number
480065

References

Reference overview
Degterev, A., et al. 2013. Nat. Chem. Biol. 9, 192.
Degterev, A., et al. 2012. Cell Death Differ. 20, 366.
Christofferson, D.E., et al. 2012. Cell Death Dis. 3, e320.
Takahashi, N., et al. 2012. Cell Death Dis. 3, e437.
Degterev, A., et al. 2008. Nat. Chem. Biol. 4, 313.
Degterev, A., et al. 2005. Nat. Chem. Biol. 1, 112.
Muller, A.J., et al. 2005. Nat. Med. 11, 312.
Teng, X., et al. 2005. Bioorg. Med. Chem. Lett. 15, 5039.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-April-2008 JSW
SynonymsNecrosome Inhibitor I, 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, Nec-1, Methyl-thiohydantoin-tryptophan, MTH-Trp, Receptor-Interacting Protein 1 Inhibitor I, RIPK 1 Inhibitor I, Nec1, RIP1 Inhibitor I, Necrosis Inhibitor II
DescriptionA cell-permeable, potent, and selective blocker of necroptosis (EC50 = 494 nM in FADD-deficient Jurkat cells treated with TNF-α), a nonapoptotic necrotic cell death pathway mediated by death-domain receptors (DRs) that offers neuroprotection in a murine model of ischemic brain injury. Exhibits no effect on DR-induced apoptosis. Also acts as a selective and ATP-competitive inhibitor of RIP1 kinase with negligible effect of RIP2 kinase activity. Nec-1 target appears to be a critical common necroptotic step upstream of execution events and downstream of DRs. Inactive control, Nec-1i, is also available (Cat. No. 480066).
FormYellow crystalline solid
Intert gas (Yes/No) Packaged under inert gas
CAS number4311-88-0
Chemical formulaC₁₃H₁₃N₃OS
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (10 mg/ml) or Methanol (5 mg/ml)
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesDegterev, A., et al. 2013. Nat. Chem. Biol. 9, 192.
Degterev, A., et al. 2012. Cell Death Differ. 20, 366.
Christofferson, D.E., et al. 2012. Cell Death Dis. 3, e320.
Takahashi, N., et al. 2012. Cell Death Dis. 3, e437.
Degterev, A., et al. 2008. Nat. Chem. Biol. 4, 313.
Degterev, A., et al. 2005. Nat. Chem. Biol. 1, 112.
Muller, A.J., et al. 2005. Nat. Med. 11, 312.
Teng, X., et al. 2005. Bioorg. Med. Chem. Lett. 15, 5039.