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481409 PTEN, His•Tag® and S•Tag™, Human Recombinant, S. frugiperda

481409
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
481409-10UG Plastic ampoule 10 μg
Description
OverviewFull-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His•Tag® and S•Tag sequences and expressed in S. frugiperda insect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases.
Catalogue Number481409
Brand Family Calbiochem®
SynonymsPhosphatase and Tensin Homolog Deleted on Chromosome 10
References
ReferencesHarrington, L.S., et al. 2005. Trends Biochem. Sci. 30, 35.
Osaki, M., et al. 2004. Apoptosis 9, 667.
Lennon, G., et al. 1996. Genomics 33, 151.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP₃ per min at 37°C.
FormLiquid
FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Purity≥80% by SDS-PAGE
Specific Activity≥75 U/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

PTEN, His•Tag® and S•Tag™, Human Recombinant, S. frugiperda SDS

Title

Safety Data Sheet (SDS) 

PTEN, His•Tag® and S•Tag™, Human Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
481409

References

Reference overview
Harrington, L.S., et al. 2005. Trends Biochem. Sci. 30, 35.
Osaki, M., et al. 2004. Apoptosis 9, 667.
Lennon, G., et al. 1996. Genomics 33, 151.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-June-2008 RFH
SynonymsPhosphatase and Tensin Homolog Deleted on Chromosome 10
DescriptionFull-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His•Tag® and S•Tag sequences and expressed in S. frugiperda insect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases.
FormLiquid
FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions100 mM Tris-HCl, 10 mM DTT, pH 8.0, 25 µg/ml PTEN, 37°C.
Purity≥80% by SDS-PAGE
Specific activity≥75 U/mg protein
Unit definitionOne unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP₃ per min at 37°C.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesHarrington, L.S., et al. 2005. Trends Biochem. Sci. 30, 35.
Osaki, M., et al. 2004. Apoptosis 9, 667.
Lennon, G., et al. 1996. Genomics 33, 151.