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382159 PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb

382159
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Nem, Dr, H, T, XnRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
382159-50UG Plastic ampoule 50 μg
Description
OverviewRecognizes the ~17 kDa histone H3 protein phosphorylated at Ser10.
Catalogue Number382159
Brand Family Calbiochem®
Application Data
Detection of human histone H3, phosphorylated on Ser10, by immunoprecipitation. Sample: Acid extracted proteins from HeLa cells. Primary antibody: PhosphoDetect™ Anti-Histone H3 (pSer10) (7-20) Rabbit pAb (Cat. No. 382159) (assay dependent). Detection: chemiluminescence.
References
ReferencesAjiro, K., et al. 1996. J. Biol. Chem. 271, 13197.
Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098.
Mahadevan, L.C., et al. 1991. Cell 65, 775.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Positive controlAcid-extracted proteins from colcemid-arrested HeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoprecipitation
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunohistochemistry
Application NotesImmunoblotting (0.5 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunohistochemistry (see comments)
Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
Application CommentsFor immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Nematode
  • Drosophila
  • Human
  • Tetrahymena
  • Xenopus
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb Certificates of Analysis

TitleLot Number
382159

References

Reference overview
Ajiro, K., et al. 1996. J. Biol. Chem. 271, 13197.
Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098.
Mahadevan, L.C., et al. 1991. Cell 65, 775.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-August-2007 RFH
ApplicationImmunoblotting (0.5 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunohistochemistry (see comments)
Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
Application Data
Detection of human histone H3, phosphorylated on Ser10, by immunoprecipitation. Sample: Acid extracted proteins from HeLa cells. Primary antibody: PhosphoDetect™ Anti-Histone H3 (pSer10) (7-20) Rabbit pAb (Cat. No. 382159) (assay dependent). Detection: chemiluminescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser10.
BackgroundHistone H1 and H3 phosphorylations correlate with the process of chromosome condensation. H3 mRNA-positive cells are most often found in tissues with a high proliferation rate, such as basal cells of the skin and crypt cells of the intestine.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH
IsotypeIgG
SpeciesC. elegans, Drosophila, Tetrahymena, Xenopus, human
Positive controlAcid-extracted proteins from colcemid-arrested HeLa cells
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsFor immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesAjiro, K., et al. 1996. J. Biol. Chem. 271, 13197.
Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098.
Mahadevan, L.C., et al. 1991. Cell 65, 775.