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480150 Ras-Net (Elk-3) Pathway Inhibitor, XRP44X - CAS 729605-21-4 - Calbiochem

480150
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
729605-21-4C₂₁H₂₁ClN₄O

Products

Catalogue NumberPackaging Qty/Pack
480150-5MG 5 mg
Description
OverviewA cell-permeable pyrazole compound that is shown to prevent in vitro tubulin polymerization (IC50 = 0.2 µM) by targeting Colchicine (Cat. No. 234115) binding site (Kd = 0.3 µM) in cell-free assays and induce blebs formation in HUVEC cultures by perturbing both tubulin microtubles and actin cytoskeleton structures. Unrelated to its effects on actin and tubulin filaments, XRP44X is also reported to block the activations of Ras and its downstream effector molecules, such as Raf, MEK, Erk, Rsk, and Net (Elk-3/SAP-2/Erp), upon FGF-2 (Cat. No. 341595) stimulation. Potently inhibits the growth of HUVEC, NIH3T3, as well as 9 other cancer cell lines in antiproliferation assays (IC50 ≤11.3 nM).
Catalogue Number480150
Brand Family Calbiochem®
SynonymsActin Assembly Inhibitor XVI, Ras Activation Inhibitor, Tubulin Polymerization Inhibitor IX, 1-(3-Chlorophenyl)-4-((3-methyl-1-phenyl-1H-pyrazol-5-yl)carbonyl)piperazine
References
ReferencesWasylyk, C., et al. 2008. Cancer Res. 68, 1275.
Product Information
CAS number729605-21-4
FormWhite solid
Hill FormulaC₂₁H₂₁ClN₄O
Chemical formulaC₂₁H₂₁ClN₄O
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥98% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
S PhraseS: 22-24/25-36/37/39

Do not breathe dust.
Avoid contact with skin and eyes.
Wear suitable protective clothing, gloves and eye/face protection.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Ras-Net (Elk-3) Pathway Inhibitor, XRP44X - CAS 729605-21-4 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Ras-Net (Elk-3) Pathway Inhibitor, XRP44X - CAS 729605-21-4 - Calbiochem Certificates of Analysis

TitleLot Number
480150

References

Reference overview
Wasylyk, C., et al. 2008. Cancer Res. 68, 1275.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-February-2010 RFH
SynonymsActin Assembly Inhibitor XVI, Ras Activation Inhibitor, Tubulin Polymerization Inhibitor IX, 1-(3-Chlorophenyl)-4-((3-methyl-1-phenyl-1H-pyrazol-5-yl)carbonyl)piperazine
DescriptionA cell-permeable pyrazole compound that is shown to prevent in vitro tubulin polymerization (IC50 = 0.2 µM) by targeting Colchicine (Cat. No. 234115) binding site (Kd = 0.3 µM) in cell-free assays and induce blebs formation in HUVEC cultures by perturbing both tubulin microtubles and actin cytoskeleton structures. Unrelated to its effects on actin and tubulin filaments, XRP44X is also reported to block the activations of Ras and its downstream effector molecules, such as Raf, MEK, Erk, Rsk, and Net (Elk-3/SAP-2/Erp), upon FGF-2 (Cat. No. 341595) stimulation. Potently inhibits the growth of HUVEC, NIH3T3, as well as 9 other cancer cell lines in antiproliferation assays (IC50 ≤11.3 nM).
FormWhite solid
Intert gas (Yes/No) Packaged under inert gas
CAS number729605-21-4
Chemical formulaC₂₁H₂₁ClN₄O
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (100 mM) or Ethanol (50 mM)
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesWasylyk, C., et al. 2008. Cancer Res. 68, 1275.